The name Hartslock is believed to derive from the Hart family, who in the 15th century owned a 'lock', probably a wooden construction used to support nets and eel traps, across the river below the woods. This structure no longer exists, having been removed in stages during the 19th and early 20th centuries. In 1915, Hartslock Wood was one of the sites listed by Charles Rothschild, the founder of the Wildlife Trusts, as "worthy of preservation", although the exigencies of the First World War meant that no practical steps were taken to protect it then. In the 1950s and 60s, local residents reacted to encroaching agriculture by helping to monitor and protect the orchids, and in 1975/76 BBOWT bought part of the site and established it as a nature reserve. In 1986, the whole site was designated as an SSSI.[1][6][7]
The Thames Path passes through Hartslock Woods. The path is usually at some distance from the river, as this is a stretch of the path that is not based on the old Thames towpath, which is on the opposite bank at this point.