HCT116 cells

HCT116 cells stained for γ‐H2AX (green) and telomeric DNA (red)

HCT116 is a human colon cancer cell line used in therapeutic research and drug screenings.[1]

Characteristics

HCT116 cells have a mutation in codon 13 of the KRAS proto-oncogene, and are suitable transfection targets for gene therapy research.[2] The cells have an epithelial morphology and can metastasize in xenograft models.[1] When transducted with viral vectors carrying the p53 gene, HCT116 cells remain arrested in the G1 phase.[3] The proliferation of HCT116 colonies was found to be inhibited by 5-Fu/P85 copolymer micelles.[4] Furthermore, it was found that the knockout of MARCH2 limited growth of HCT116 cells via stress on the endoplasmic reticulum.[5]

Use in research

HCT116 cells are used in a variety of biomedical studies involving colon cancer proliferation and corresponding inhibitors. The cell line has been used in tumorigenicity studies, along with other research that has shown that Cyclin D1 holds large importance for the activity of lithocholic acid hydroxyamide. HCT116 cells can also function in xenografts, with docetaxel, 5-FU, and flavopiridol limiting tumor growth in vitro.

The HCT116 cell line was found to have two variations; one with a large expression of the Insp8 gene, and the other without. The Insp8 gene is part of a cell's energy metabolism process, and can affect the cellular phenotype as a result.[6]

References

  1. ^ a b Rajput, Ashwani; et al. (15 June 2008). "Characterization of HCT116 Human Colon Cancer Cells in an Orthotopic Model". Journal of Surgical Research. 147 (2): 276–281. doi:10.1016/j.jss.2007.04.021. PMID 17961596.
  2. ^ "HCT 116". ATCC. Retrieved 4 May 2018.
  3. ^ Kaeser, Matthias; et al. (9 December 2003). "Regulation of p53 Stability and Function in HCT116 Colon Cancer Cells". Journal of Biological Chemistry. 279 (9): 7598–7605. doi:10.1074/jbc.M311732200. PMID 14665630.
  4. ^ Zhu, Pengxi (11 February 2016). "Inhibition of Growth and Metastasis of Colon Cancer by Delivering 5-Fluorouracil-loaded Pluronic P85 Copolymer Micelles". Scientific Reports. 6: 20896. Bibcode:2016NatSR...620896Z. doi:10.1038/srep20896. PMC 4750032. PMID 26864651.
  5. ^ Xia, Dan (27 July 2017). "Knockout of MARCH2 inhibits the growth of HCT116 colon cancer cells by inducing endoplasmic reticulum stress". Cell Death and Disease. 8 (7): e2957. doi:10.1038/cddis.2017.347. PMC 5584615. PMID 28749466.
  6. ^ Gu, Chunfang (27 October 2016). "Inositol Pyrophosphate Profiling of Two HCT116 Cell Lines Uncovers Variation in InsP8 Levels". PLOS ONE. 11 (10): e0165286. Bibcode:2016PLoSO..1165286G. doi:10.1371/journal.pone.0165286. PMC 5082907. PMID 27788189.


Stub icon

This oncology article is a stub. You can help Wikipedia by expanding it.

Stub icon

This genetics article is a stub. You can help Wikipedia by expanding it.

Read other articles:

List of cities in Australia with the most skyscrapers

Australia has over 130 skyscrapers —buildings which reach or exceed heights of 150 metres (490 ft))— complete and or under construction, all spread throughout five cities of the country. Most skyscrapers are located in cities within the Eastern states of Australia, and a number of these cities, such as Melbourne and Sydney, also rank high in the world list of cities with the most skyscrapers. Cities by number of skyscrapers Five cities in Australia currently host at least one skyscr...

 

Mayor of Liverpool

Chief executive of Liverpool, England For the ceremonial office of Lord Mayor of Liverpool, see Lord Mayor of Liverpool. For the Metro Mayor of the Liverpool Region, see Metro Mayor of the Liverpool City Region. Mayor of LiverpoolArms of Liverpool City CouncilStyleNo titleAppointerElectorate of LiverpoolTerm lengthFour yearsInaugural holderJoe AndersonFormation7 May 2012Final holderJoanne AndersonSuccessionLeader of Liverpool City CouncilDeputyDeputy Mayor of Liverpool From 2012 to 2023, the ...

 

Billy Ocean

Billy OceanInformasi latar belakangNama lahirLeslie Sebastian CharlesLahir21 Januari 1950 (umur 74)Fyzabad, TrinidadGenreR&B, pop, soulPekerjaanPenulis lagu, musisi, penyanyiInstrumenVocals, steel drums, gitarTahun aktif1972–sekarangLabelGTO, Epic, Jive, Aqua MusicSitus webwww.billyocean.co.uk Leslie Sebastian Charles atau dikenal sebagai Billy Ocean, (lahir 21 Januari 1950) adalah penyanyi berkebangsaan Inggris yang lahir di Trinidad. Album Judul Detail album Posisi di chart Serti...

Tyrone Power

Tyrone Power Tyrone Power (5 Mei 1914-15 November 1958) adalah aktor Amerika yang sangat terkenal pada zamannya.[1] Ia berasal dari keluarga yang berkecimpung di dunia teater dan selama beberapa waktu terjun ke bidang tersebut.[1] Lalu, ia pindah ke bidang radio bersama dengan Don Amache dan kembali lagi ke panggung sandiwara.[1] Film-filmnya yang terkenal antara lain Tom Brown of Culver (1932), Ladies in Love (1936), In Old Chicagao (1938), The Raine Came (1939), Mark...

 

Dommeldange

Cet article est une ébauche concernant une localité luxembourgeoise. Vous pouvez partager vos connaissances en l’améliorant (comment ?) selon les recommandations des projets correspondants. Dommeldange (lb) Dummeldeng (de) Dommeldingen La gare. Administration Pays Luxembourg Canton Luxembourg Commune Luxembourg Démographie Population 2 951 hab.[1] (31 décembre 2023) Densité 1 253 hab./km2 Géographie Coordonnées 49° 38′ 02″ nord, 6° ...

 

2014 European Parliament election in Sweden

European Parliament election in Sweden of 2014 2014 European Parliament election in Sweden ← 2009 25 May 2014 2019 → All 20 Swedish seats to the European ParliamentTurnout51.07% ( 5.54 pp)[1]   First party Second party Third party   Leader Marita Ulvskog Isabella Lövin Gunnar Hökmark Party Social Democrats Green Moderate Alliance S&D Greens/EFA EPP Last election 6 seats, 24.4% 2 seats, 11.0% 4 seats, 18.8% Seats won 5 4 3 Seat chang...

Carlos Arroyo

Carlos Arroyo Arroyo con la maglia del Galatasaray Nazionalità  Porto Rico Altezza 188 cm Peso 92 kg Pallacanestro Ruolo Playmaker Termine carriera 2019 Carriera Giovanili 1995-1997 Cang. de Santurce1997-2001 FIU Panthers Squadre di club 1996-1997 Carid. de Fajardo66 (914)1998-2001 Cang. de Santurce95 (1344)2001-2002 Toronto Raptors17 (30)2002 Saski Baskonia4 (17)2002 Denver Nuggets20 (81)2002 Cang. de Santurce10 (200)2002-2005 Utah Jazz145 ...

 

Festa del Litorale sloveno

Questa voce o sezione sull'argomento festività non cita le fonti necessarie o quelle presenti sono insufficienti. Puoi migliorare questa voce aggiungendo citazioni da fonti attendibili secondo le linee guida sull'uso delle fonti. Segui i suggerimenti del progetto di riferimento. Festa del Litorale slovenoLa regione storica del Litorale sloveno all'interno della SloveniaTiponazionale Data15 settembre Celebrata in Slovenia Celebrata aSoprattutto nel Litorale sloveno e a Lubiana Ogge...

 

Norgesmesterskapet 1937

Norgesmesterskapet 1937NM menn 1937 Competizione Coppa di Norvegia Sport Calcio Edizione 36ª Organizzatore NFF Luogo  Norvegia Risultati Vincitore  Mjøndalen(3º titolo) Secondo  Odd Cronologia della competizione 1936 1938 Manuale La Norgesmesterskapet 1937 di calcio fu la 36ª edizione del torneo. La squadra vincitrice fu il Mjøndalen, che vinse la finale contro l'Odd con il punteggio di 4-2. Indice 1 Risultati 1.1 Terzo turno 1.1.1 Ripetizione 1.2 Quarto turno 1.2.1 Ripet...

Египтопитек

† Египтопитек Реконструкция внешнего вида египтопитека Научная классификация Домен:ЭукариотыЦарство:ЖивотныеПодцарство:ЭуметазоиБез ранга:Двусторонне-симметричныеБез ранга:ВторичноротыеТип:ХордовыеПодтип:ПозвоночныеИнфратип:ЧелюстноротыеНадкласс:Четвероно...

 

Politeknik Penerbangan Surabaya

Politeknik Penerbangan Surabaya Politeknik Penerbangan Surabaya adalah pendidikan tinggi di bawah Kementerian Perhubungan Indonesia, dengan tugas pokok melaksanakan pendidikan profesional program diploma bidang keahlian teknik dan keselamatan penerbangan yang terbuka bagi umum. Sejarah Berkas:POLTEKBANG SURABAYA HWeb.jpgGedung Utama Keberadaan Poltekbang Surabaya diawali dengan pemanfaatan aset Kantor Wilayah III Direktorat Jenderal Perhubungan Udara Surabaya pada tahun 1989 dengan nama Pusdi...

 

哈萨克斯坦总统

此条目序言章节没有充分总结全文内容要点。 (2019年3月21日)请考虑扩充序言，清晰概述条目所有重點。请在条目的讨论页讨论此问题。 哈萨克斯坦總統哈薩克總統旗現任Қасым-Жомарт Кемелұлы Тоқаев卡瑟姆若马尔特·托卡耶夫自2019年3月20日在任任期7年首任努尔苏丹·纳扎尔巴耶夫设立1990年4月24日（哈薩克蘇維埃社會主義共和國總統） 哈萨克斯坦 哈萨克斯坦政府...

Melanie C (album)

2020 studio album by Melanie CMelanie CStudio album by Melanie CReleased2 October 2020 (2020-10-02)Recorded2019–2020Genre Electronic pop Length35:03LabelRed Girl MediaProducer Billen Ted Fryars Future Cut Alex Metric Paul O'Duffy One Bit George Reid Biff Stannard Ten Ven Melanie C chronology Version of Me(2016) Melanie C(2020) Singles from Melanie C Who I AmReleased: 13 March 2020 Blame It on MeReleased: 27 May 2020 In and Out of LoveReleased: 29 July 2020 FearlessRe...

 

Mega Man (character)

Video game character Fictional character Mega ManMega Man characterArtwork of Mega Man from Mega Man X DiVEFirst appearanceMega Man (1987)Created byAkira KitamuraDesigned byAkira KitamuraKeiji InafuneVoiced by English Doug Parker(Captain N: The Game Master)Ian James Corlett(MM 1994 TV series)Gregory Smith(MM: Upon a Star)Ruth Marie Jarman (MM8)[1]Cole Howard(MMPU)[2]Chris Cason(Street Fighter X Tekken)[2]Benjamin Diskin(MM11)[2]Vincent Tong(MM: Fully Charged) J...

 

Museum Jerman

Deutsches MuseumDeutsches Museum dengan Jembatan BoschDidirikan28 Juni 1903 (1903-06-28)LokasiMuseumsinsel 180538 MünchenGermanySitus webMuseum Jerman Deutsches Museum (Yang berarti Museum Jerman) (bahasa Jerman: Deutsches Museum atau Das Deutsche Museum) terletak di München, Jerman, dan merupakan museum ilmu pengetahuan dan teknologi terbesar di dunia,[1] dengan pengunjung sekitar 1,5 juta orang per tahun dan sekitar 28.000 benda dari 50 cabang ilmu pengetahuan dan te...

Rena Sofer

American actress (born 1968) Rena SoferSofer in 2014BornRena Sherel Sofer (1968-12-02) December 2, 1968 (age 55)Arcadia, California, U.S.OccupationActressYears active1984–presentSpouses Wally Kurth ​ ​(m. 1995; div. 1997)​ Sanford Bookstaver ​ ​(m. 2003; div. 2017)​ ​ ​(m. 2024)​ Children2 Rena Sherel Sofer (born December 2, 1968)[1] is an Ame...

 

2012年中國中央電視臺春節聯歡晚會

2012年中国中央电视台春节联欢晚会类型賀歲節目导演哈文主持朱軍、李詠、董卿、畢福劍、撒貝寧、李思思主演节目单制作国家／地区 中国大陆语言普通话每集长度4小时24分40秒片尾曲《天下一家》作曲洪兵作词陈道斌演唱王莉、廖昌永制作拍攝地點北京中央电视台彩色电视中心一号演播大厅中央电视台综合频道（CCTV-1）首播 图像制式标清576i4:3高清1080i16:9声音制式单声道�...

 

Doğubayazıt

For other uses, see Beyazid (disambiguation). Municipality in Ağrı, TurkeyDoğubayazıtMunicipalityDoğubayazıt from Ishak Pasha PalaceDoğubayazıtLocation in TurkeyCoordinates: 39°32′50″N 44°05′00″E / 39.54722°N 44.08333°E / 39.54722; 44.08333CountryTurkeyProvinceAğrıDistrictDoğubayazıtGovernment • MayorYıldız Acar (HDP)Population (2021)[1]80,061Time zoneUTC+3 (TRT)Websitewww.dogubayazit.bel.tr Ishak Pasha Palace near Do�...

Marietta, Ohio

City in Ohio, United StatesMarietta, OhioCityDowntown Marietta, with Muskingum River in foreground, and Ohio River in background rightInteractive map of MariettaMariettaShow map of OhioMariettaShow map of the United StatesCoordinates: 39°25′27″N 81°26′47″W / 39.42417°N 81.44639°W / 39.42417; -81.44639CountryUnited StatesStateOhioCountyWashington[1]SettledApril 7, 1788IncorporatedDecember 2, 1800Named forMarie Antoinette[2]Government �...

 

Łukasz Broź

Questa voce sull'argomento calciatori polacchi è solo un abbozzo. Contribuisci a migliorarla secondo le convenzioni di Wikipedia. Segui i suggerimenti del progetto di riferimento. Łukasz BroźNazionalità Polonia Altezza180 cm Calcio RuoloDifensore Squadra Mazovia Mińsk Mazowiecki CarrieraGiovanili ABC Bagbud Giżycko Squadre di club1 2004-2005 Mamry Giżycko? (?)2005-2006 Kmita Zabierzów? (?)2006-2013 Widzew Łódź187 (11)2013-2018 Legia Varsavia99 (1)2018-2020...