GalNAc-alpha-(1-4)-GalNAc-alpha-(1-3)-diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase

GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase
Identifiers
EC no.2.4.1.292
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BRENDABRENDA entry
ExPASyNiceZyme view
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MetaCycmetabolic pathway
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NCBIproteins

GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase (EC 2.4.1.292, PglH) is an enzyme with systematic name UDP-N-acetyl-alpha-D-galactosamine:GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-diNAcBac-PP-tritrans,heptacis-undecaprenol 4-alpha-N-acetyl-D-galactosaminyltransferase.[1][2][3] This enzyme catalyses the following chemical reaction

3 UDP-N-acetyl-alpha-D-galactosamine + GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-diNAcBac-PP-tritrans,heptacis-undecaprenol 3 UDP + [GalNAc-alpha-(1->4)]4-GalNAc-alpha-(1->3)-diNAcBac-PP-tritrans,heptacis-undecaprenol

This enzyme is solated from Campylobacter jejuni.

References

  1. ^ Glover KJ, Weerapana E, Imperiali B (October 2005). "In vitro assembly of the undecaprenylpyrophosphate-linked heptasaccharide for prokaryotic N-linked glycosylation". Proceedings of the National Academy of Sciences of the United States of America. 102 (40): 14255–9. Bibcode:2005PNAS..10214255G. doi:10.1073/pnas.0507311102. PMC 1242339. PMID 16186480.
  2. ^ Troutman JM, Imperiali B (March 2009). "Campylobacter jejuni PglH is a single active site processive polymerase that utilizes product inhibition to limit sequential glycosyl transfer reactions". Biochemistry. 48 (12): 2807–16. doi:10.1021/bi802284d. PMC 2736683. PMID 19159314.
  3. ^ Børud B, Viburiene R, Hartley MD, Paulsen BS, Egge-Jacobsen W, Imperiali B, Koomey M (June 2011). "Genetic and molecular analyses reveal an evolutionary trajectory for glycan synthesis in a bacterial protein glycosylation system". Proceedings of the National Academy of Sciences of the United States of America. 108 (23): 9643–8. Bibcode:2011PNAS..108.9643B. doi:10.1073/pnas.1103321108. PMC 3111294. PMID 21606362.