South and east of Naples, US 41 turns eastward as SR 90 as the Tamiami Trail crosses the Everglades on its way to Miami; north of High Springs, US 41 overlaps US 441 (SR 25) until their split in Lake City (from there US 41 continues to the Georgia border with the hidden SR 25 designation).
Separations of US 41 and SR 45 between SR 45 termini
State Road 45A (SR 45A) is the Venice Bypass, a segment along U.S. Route 41 (US 41) east of the Tamiami Trail in Venice, which was originally part of US 41 until 1965 when that segment was redesignated as US 41 Bus after the Intracoastal Waterway (ICW) was dredged through Venice by the U.S. Army Corps of Engineers in 1964.[2] The route begins near Shamrock Boulevard in Venice Gardens and terminates at Venetia Bay Boulevard in the Eastgate section of Venice.