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Earle's balanced salt solution is an isotonic saline solution (or balanced salt solution) formulated by W.R. Earle in 1943.^[1] It contains sodium chloride, potassium chloride, calcium chloride, magnesium sulfate, sodium dihydrogen phosphate, sodium bicarbonate and dextrose (glucose). It is intended to be used in 5% CO₂ atmosphere. It is a base of many cell culture media.^[2]

References

^ Earle, Wilton R.; Schilling, Edward L.; Stark, Thomas H.; Straus, Nancy P.; Brown, Mary F.; Shelton, Emma (1943-10-01). "Production of Malignancy in Vitro. IV. The Mouse Fibroblast Cultures and Changes Seen in the Living Cells". JNCI: Journal of the National Cancer Institute. 4 (2): 165–212. doi:10.1093/jnci/4.2.165. ISSN 0027-8874.
^ Freshney, R. Ian (2010). Culture of animal cells : a manual of basic technique and specialized applications (6th ed.). Hoboken, N.J.: Wiley-Blackwell. ISBN 978-0-470-64936-7. OCLC 711779369.

[Earle-1] Earle, Wilton R.; Schilling, Edward L.; Stark, Thomas H.; Straus, Nancy P.; Brown, Mary F.; Shelton, Emma (1943-10-01). "Production of Malignancy in Vitro. IV. The Mouse Fibroblast Cultures and Changes Seen in the Living Cells". JNCI: Journal of the National Cancer Institute. 4 (2): 165–212. doi:10.1093/jnci/4.2.165. ISSN 0027-8874.

[Fresh-2] Freshney, R. Ian (2010). Culture of animal cells : a manual of basic technique and specialized applications (6th ed.). Hoboken, N.J.: Wiley-Blackwell. ISBN 978-0-470-64936-7. OCLC 711779369.

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