The thirty-second running of the Preakness Stakes was held on Tuesday, May 21, 1907. On that day Don Enrique went off as 15-1 longshot in the field of seven after three colts scratched. In the race, he broke poorly in fourth place under jockey George Mountain but as they reached Pimlico's famous "Clubhouse Turn" he had edged up into third place. The pace was moderate with the first quarter run in :24-1/5 seconds and the half in :48-4/5.[4] As the race progressed, Don Enrique was part of a group of stalkers behind the leaders. He made his move on the final turn with some urging from Mountain and rounded the field.
Near the top of the lane he passed most of the leaders and wore down front runner Ethon. In the last sixteenth of a mile he emerged in front and beat the second favorite and now runner-up by only one length.[5] That day Ethon finished second at 3-1 way out in front over the favorite Zambesi by three lengths who finished third at 2-1.
The final time for the one mile and 70 yard race on dirt was 1:45-2/5 over a heavy track. Don Enrique won almost half of the total purse $5,000 netting earnings $2,260.[6]
Later racing career
Later in his three-year-old season Don Enrique won the six furlong Ravenswood Handicap at Jamaica Race Course. He also finished third in the mile and one eighth Saranac Handicap at Saratoga Race Course.