In enzymology, a deacetylcephalosporin-C acetyltransferase (EC 2.3.1.175) is an enzyme that catalyzes the chemical reaction

acetyl-CoA + deacetylcephalosporin C ${\displaystyle \rightleftharpoons }$ CoA + cephalosporin C

Thus, the two substrates of this enzyme are acetyl-CoA and deacetylcephalosporin C, whereas its two products are CoA and cephalosporin C.

This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. The systematic name of this enzyme class is acetyl-CoA:deacetylcephalosporin-C O-acetyltransferase. Other names in common use include acetyl-CoA:deacetylcephalosporin-C acetyltransferase, DAC acetyltransferase, cefG, deacetylcephalosporin C acetyltransferase, acetyl coenzyme A:DAC acetyltransferase, acetyl-CoA:DAC acetyltransferase, CPC acetylhydrolase, acetyl-CoA:DAC O-acetyltransferase, and DAC-AT. This enzyme participates in penicillin and cephalosporin biosynthesis.

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• Gutierrez S, Velasco J, Fernandez FJ, Martin JF (1992). "The cefG gene of Cephalosporium acremonium is linked to the cefEF gene and encodes a deacetylcephalosporin C acetyltransferase closely related to homoserine O-acetyltransferase". J. Bacteriol. 174 (9): 3056–64. PMC 205961. PMID 1569032.
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