DSB may refer to:

• Dictionary of Scientific Biography, a multivolume reference work edited by Charles Coulston Gillespie
• Double strand break, a break in both DNA strands, part of DNA repair
• Double-sideband transmission, in telecommunications
• Dsb, the warm-summer Mediterranean continental climate in the Köppen climate classification
• DsbA, a bacterial member of the Dsb (disulfide bond) family of enzymes

• De Surinaamsche Bank, Suriname
• Defense Science Board, of the United States
• Democrats for a Strong Bulgaria, a Bulgarian political party
• Department of Internal Security (ru:Главное управление собственной безопасности МВД России) or Departamenta sobstvennoi bezopasnosti of the Russian Ministry of Internal Affairs, an anticorruption and internal crime detection agency in Russia
• Deutsche Schallplatten Berlin, another name of VEB Deutsche Schallplatten
• Deutsche Schule Bratislava, a German international school in Bratislava, Slovakia
• Deutscher Schützenbund, the German Shooting and Archery Federation
• Dispute Settlement Body, of the World Trade Organization
• DSB (railway company) (Danske Statsbaner), a Danish train operating company
• DSB Bank (Dirk Scheringa Beheer), Netherlands
  • DSB Stadion, a football stadium in Alkmaar, Netherlands sponsored by DSB Bank
• DSB International School, a German school in Mumbai, India
• Dutch Safety Board, a Netherlands investigation agency
• German International School of Cairo (Deutsche Schule der Borromäerinnen Kairo), Egypt
• German School Beirut (Deutsche Schule Beirut), Lebanon
• Norwegian Directorate for Civil Protection (Direktoratet for samfunnssikkerhet og beredskap), a Norwegian government organization

• Dark square bishop, in chess
• Department Store Battles, a modification for Battlefield 2, a computer game
• Domestic Stability Buffer, a capital buffer set by a financial regulator in Canada requiring major domestic banks to keep some loan funds in reserve
• Don Salvador Benedicto, a municipality in the province of Negros Occidental, Philippines
• "Don't Stop Believin'", a song by American rock band Journey
• DSB, the ISO 639-2 or SIL International language code for the Lower Sorbian language - Dolnoserbski

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