Class 1000 may refer to:

• Class 1000 Shinkansen, a class of Japanese trains built in the 1960s
• NS Class 1000, a class of Dutch electric locomotives built in the 1940s
• Korail Class 1000, a class of South Korean trains built from 1974 to 1996
• Midland Railway 1000 Class, a class of British steam locomotives first built in 1902
• GWR 1000 Class, a British steam locomotive built in the 1940s
• LRTA 1000 class, 1980s light rail vehicles operated by the Light Rail Manila Corporation
• Class 1000 (standard), a cleanroom standard for air purity

This disambiguation page lists articles associated with the title Class 1000.
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