Protein family
In molecular biology, the choline/ethanolamine kinase family includes choline kinase (EC 2.7.1.32 ) and ethanolamine kinase (EC 2.7.1.82 ).
Ethanolamine and choline are major membrane phospholipids , in the form of glycerophosphoethanolamine and glycerophosphocholine . Ethanolamine is also a component of the glycosylphosphatidylinositol (GPI) anchor, which is necessary for cell-surface protein attachment.[ 1] de novo synthesis of these phospholipids begins with the creation of phosphoethanolamine and phosphocholine by ethanolamine and choline kinases in the first step of the CDP-ethanolamine pathway.[ 2] [ 3] Trypanosoma brucei genome .
Ethanolamine kinase has no choline kinase activity [ 1] ADP .[ 3] Inositol supplementation represses ethanolamine kinase, decreasing the incorporation of ethanolamine into the CDP-ethanolamine pathway and into phosphatidylethanolamine and phosphatidylcholine .[ 4]
References
^ a b Gibellini F, Hunter WN, Smith TK (October 2008). "Biochemical characterization of the initial steps of the Kennedy pathway in Trypanosoma brucei: the ethanolamine and choline kinases" . Biochem. J . 415 (1): 135– 44. doi :10.1042/BJ20080435 . PMC 2552378 PMID 18489261 . ^ Tian Y, Jackson P, Gunter C, Wang J, Rock CO, Jackowski S (September 2006). "Placental thrombosis and spontaneous fetal death in mice deficient in ethanolamine kinase 2" . J. Biol. Chem . 281 (38): 28438– 49. doi :10.1074/jbc.M605861200 PMID 16861741 . ^ a b Kim KH, Voelker DR, Flocco MT, Carman GM (March 1998). "Expression, purification, and characterization of choline kinase, product of the CKI gene from Saccharomyces cerevisiae" . J. Biol. Chem . 273 (12): 6844– 52. doi :10.1074/jbc.273.12.6844 PMID 9506987 . ^ Kersting MC, Choi HS, Carman GM (August 2004). "Regulation of the yeast EKI1-encoded ethanolamine kinase by inositol and choline" . J. Biol. Chem . 279 (34): 35353– 9. doi :10.1074/jbc.M405704200 PMID 15201274 .
