Chocolate agar (CHOC) or chocolate blood agar (CBA) is a nonselective, enriched growth medium used for isolation of pathogenic bacteria.[1][2][3] It is a variant of the blood agar plate, containing red blood cells that have been lysed by slowly heating to 80°C. Chocolate agar is used for growing fastidious respiratory bacteria, such as Haemophilus influenzae and Neisseria meningitidis.[4] In addition, some of these bacteria, most notably H. influenzae, need growth factors such as nicotinamide adenine dinucleotide (factor V or NAD) and hemin (factor X), which are inside red blood cells; thus, a prerequisite to growth for these bacteria is the presence of red blood cell lysates. The heat also inactivates enzymes which could otherwise degrade NAD. The agar is named for its color and contains no chocolate products.
Chocolate agar with the addition of bacitracin becomes selective for the genus Haemophilus. Another variant of chocolate agar called Thayer–Martin agar contains an assortment of antibiotics which select for Neisseria species.
The composition of chocolate agar includes the following components:[5]
The exact concentrations of these ingredients may vary slightly depending on the specific formulation used in different laboratories or by different manufacturers.
Addition of heated blood to media was first documented for use by Cohen and Fitzgerald in 1910 and then by Dr. Olga Povitzky at the New York City Department of Health Bureau of Laboratories.[6][7] The term "chocolate agar" comes from the brown color generated from the higher concentration of heated blood in the mixture and was given the distinctive description first by Warren Crowe in 1915.[8]
This microbiology-related article is a stub. You can help Wikipedia by expanding it.
Lago Buenos Aires / General Carrera / Chelenko Vista del lagoUbicación geográficaCordillera Andes patagónicosRegión PatagoniaCoordenadas 46°26′15″S 71°42′54″O / -46.4375, -71.715Ubicación administrativaPaís Chile y ArgentinaDivisión Región de Aysén del General Carlos Ibáñez del Campo y Santa CruzSubdivisión Departamento Lago Buenos Aires (ARG)Provincia General Carrera (CHI)Cuerpo de aguaOrigen Lago glacialAfluentes Ríos Soler, Los Antiguos, Jeinemeni, Ibá
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МуниципалитетМуниципалитет Майпуисп. Partido de Maipú 36°53′ ю. ш. 57°35′ з. д.HGЯO Страна Аргентина Входит в провинцию Буэнос-Айрес Адм. центр Майпу История и география Площадь 2640 км² Высота 6 м Население Население 10 188 чел. (2010) Плотность 3,86 чел./км² Офи
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