Charlotte Harbor may refer to:

• Charlotte Harbor, Florida, a census-designated place in Charlotte County, Florida, United States
• Charlotte Harbor (estuary), a large bay on the southwest coast of Florida
• Charlotte Harbor Light, a former lighthouse in Charlotte Harbor
• Charlotte Harbor Preserve State Park, a Florida State Park composed of islands and land that surround the Charlotte Harbor estuary
• Charlotte Harbor and Northern Railway, a former railroad in southwest Florida
  • Charlotte Harbor and Northern Railway Depot, a historic railroad depot in Boca Grande, Florida

This disambiguation page lists articles associated with the title Charlotte Harbor.
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