Bismarck brown Y

Bismarck brown Y
Names
Other names
Bismarck brown
Manchester brown
Phenylene brown
Basic Brown 1
C.I. 21000
Vesuvine BA
Identifiers
3D model (JSmol)
ChemSpider
ECHA InfoCard 100.030.273 Edit this at Wikidata
  • InChI=1S/C18H18N8/c19-11-4-6-17(15(21)8-11)25-23-13-2-1-3-14(10-13)24-26-18-7-5-12(20)9-16(18)22/h1-10H,19-22H2/b25-23+,26-24+ ☒N
    Key: BDFZFGDTHFGWRQ-OGGGYYITSA-N ☒N
  • InChI=1/C18H18N8/c19-11-4-6-17(15(21)8-11)25-23-13-2-1-3-14(10-13)24-26-18-7-5-12(20)9-16(18)22/h1-10H,19-22H2/b25-23+,26-24+
    Key: BDFZFGDTHFGWRQ-OGGGYYITBR
  • CC1=C(C=C(C=C1)N=NC2=C(C=C(C(=C2)C)N)N)N=NC3=C(C=C(C(=C3)C)N)N
  • N(=N/c1ccc(cc1N)N)\c3cccc(/N=N/c2ccc(N)cc2N)c3
Properties
C18H18N8·2HCl
Molar mass 419.31 g/mol
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
☒N verify (what is checkY☒N ?)

Bismarck brown Y also called C.I. 21000[1] and C.I. Basic Brown 1,[1] is a diazo dye with the idealized formula [(H2N)2C6H3N2]2C6H4. The dye is a mixture of closely related compounds. It was one of the earliest azo dyes, being described in 1863 by German chemist Carl Alexander von Martius. It is used in histology for staining tissues.[2]

Synthesis

The dye is simple to prepare because the diamine serves both as a source of the diazonium cation and as the coupling partner in the azo coupling reaction. The synthesis is thought to start with double diazotization of 1,3-phenylenediamine:

(H2N)2C6H4 + 2 H+ + 2 HNO2 → [C6H4(N2)2]2+ + 2 H2O

It is assumed that this bis(diazonium) ion subsequently attacks two equivalents of 1,3-phenylenediamine:

2 (H2N)2C6H4 + [C6H4(N2)2]2+ → 2 H+ + [(H2N)2C6H3N2]2C6H4

In some cases, toluenediamines are used in addition to the phenylenediamine. Furthermore, the resulting dye is thought to consist of oligomers with three or more diazo groups.[2]

Uses

Bismarck brown Y stains acid mucins to yellow color. It also stains mast cell granules brown.[3] It can be used with live cells. It is also used to stain cartilage in bone specimens, as one of Kasten's Schiff-type reagents in the periodic acid-Schiff stain to stain cellulose, and in Feulgen stain to stain DNA. It was more common in the past; today it is partially replaced by other stains. It has also been used to give soap an amber color in the past.[4]

Bismarck brown Y is a constituent of Papanicolaou stain.[1]

It can also be used as a counterstain for Victoria blue R for staining of acid-fast microorganisms.

References

  1. ^ a b c Lillie, Ralph Dougall (1977). H. J. Conn's Biological stains (9th ed.). Baltimore: Williams & Wilkins. pp. 692p.
  2. ^ a b Booth, Gerald (2000). "Dyes, General Survey". Ullmann's Encyclopedia of Industrial Chemistry. Weinheim: Wiley-VCH. doi:10.1002/14356007.a09_073. ISBN 3527306730.
  3. ^ Tomov, Nikola; Dimitrov, Nikolay (2017). "Modified Bismarck brown staining for demonstration of soft tissue mast cells" (PDF). Trakia Journal of Sciences. 15 (3): 195–197. doi:10.15547/tjs.2017.03.001.
  4. ^ The Chemical Formulary. David Van Nostrand Company Inc. 1933. p. 86.

Read other articles:

List of official overseas trips made by William, Prince of Wales, and Catherine, Princess of Wales

The Prince and Princess of Wales (then known as the Duke and Duchess of Cambridge) in Ottawa, Canada during their first joint royal tour outside the United Kingdom from 30 June to 8 July 2011 This is a list of official overseas visits and Commonwealth tours made by the Prince and Princess of Wales. Prince William, as a child, first traveled with his parents on official visits and tours. He is presently one of the United Kingdom's most important ambassadors; sometimes the Prince travels overs...

 

الدوري النمساوي 1983–84

يفتقر محتوى هذه المقالة إلى الاستشهاد بمصادر. فضلاً، ساهم في تطوير هذه المقالة من خلال إضافة مصادر موثوق بها. أي معلومات غير موثقة يمكن التشكيك بها وإزالتها. (نوفمبر 2019) الدوري النمساوي 1983–84 تفاصيل الموسم الدوري النمساوي  النسخة 73  البلد النمسا  المنظم اتحاد النمسا ...

 

Pemilihan umum Gubernur Sulawesi Utara 2020

Pemilihan Umum Gubernur Sulawesi Utara 2020201520249 Desember 2020[1]Kandidat   Calon Olly Dondokambey Christiany Eugenia Paruntu Vonnie Anneke Panambunan Partai PDI-P Partai Golongan Karya NasDem Aliansi Daftar PDI-P Gerindra PKB PSI Daftar Golkar PAN Demokrat Pendamping Steven Kandouw Sehan Salim Landjar Hendry Corneles Mamengko Runtuwene Suara rakyat 821.503 491.457 125.627 Persentase 57.1% 34.16% 8.73% Logo Peta persebaran suara Peta Sulawesi yang menyoroti Sulawesi Uta...

المرأة في الفلسفة

جزء من سلسلةالمرأة في المجتمع مجتمع قطاع الأعمال التعليم القوى العاملة (مجالس الإدارة) السياسة (في أستراليا) الخدمة العسكرية للمرأة في التاريخ الحقوق القانونية التاريخ جائزة نوبل حقوق الحيوانات العلوم والتكنولوجيا الطب العلوم الهندسة الحوسبة الفنون والعلوم الإنسانية ال...

 

Divisi Kriminal Departemen Kehakiman Amerika Serikat

Artikel ini perlu diwikifikasi agar memenuhi standar kualitas Wikipedia. Anda dapat memberikan bantuan berupa penambahan pranala dalam, atau dengan merapikan tata letak dari artikel ini. Untuk keterangan lebih lanjut, klik [tampil] di bagian kanan. Mengganti markah HTML dengan markah wiki bila dimungkinkan. Tambahkan pranala wiki. Bila dirasa perlu, buatlah pautan ke artikel wiki lainnya dengan cara menambahkan [[ dan ]] pada kata yang bersangkutan (lihat WP:LINK untuk keterangan lebih lanjut...

 

Instability strip

Region of an astronomical diagram For the S Doradus Instability Strip, see Luminous blue variable. The unqualified term instability strip usually refers to a region of the Hertzsprung–Russell diagram largely occupied by several related classes of pulsating variable stars:[1] Delta Scuti variables, SX Phoenicis variables, and rapidly oscillating Ap stars (roAps) near the main sequence; RR Lyrae variables where it intersects the horizontal branch; and the Cepheid variables where it cr...

Pursat River

River in Pursat Province, CambodiaPursat Riverស្ទឹងពោធិ៍សាត់Sampov Meas Island on the Pursat RiverLocationCountryCambodiaProvincePursat ProvincePhysical characteristicsMouth  • locationTonle Sap The Pursat River (Khmer: ស្ទឹងពោធិ៍សាត់, Steung Pursat) also known as Steung Tamyong is a major river of western Cambodia, It drains about two-thirds of the chain to the Tonlé Sap.[1] Pursat and Tumpor lie along...

 

1738 en science

Chronologies Données clés 1735 1736 1737  1738  1739 1740 1741Décennies :1700 1710 1720  1730  1740 1750 1760Siècles :XVIe XVIIe  XVIIIe  XIXe XXeMillénaires :-Ier Ier  IIe  IIIe Chronologies thématiques Art Architecture, Arts plastiques (Dessin, Gravure, Peinture et Sculpture), Littérature (), Musique classique et Théâtre   Ingénierie (), Architecture et ()   Politique Droit   Religion (,)   Science () et S...

 

Texas A&M–Commerce Lions women's soccer

American college soccer team Texas A&M–Commerce Lions women's soccerFounded1995UniversityTexas A&M University–CommerceHead coachAshley Gordon (2nd season)ConferenceSouthlandLocationCommerce, TexasStadiumLion Soccer Field (Capacity: 500)NicknameLionsColorsBlue and gold[1]    Home Away NCAA Tournament Quarterfinals1999NCAA Tournament appearances1999, 2003, 2004, 2008, 2013, 2014, 2015, 2016Conference Tournament championships1999, 2004, 2014, 2015Conf...

Linagliptin

Chemical compound LinagliptinClinical dataPronunciation/ˌlɪnəˈɡlɪptɪn/ LIN-ə-GLIP-tin Trade namesTradjenta, Trajenta, TrazentaOther namesBI-1356AHFS/Drugs.comMonographMedlinePlusa611036License data US DailyMed: Linagliptin Routes ofadministrationBy mouthATC codeA10BH05 (WHO) Legal statusLegal status AU: S4 (Prescription only)[2][3] CA: ℞-only[4] UK: POM (Prescription only)[5] US: WARNING[1]Rx-only&...

 

Only the Young (Taylor Swift song)

2020 promotional single by Taylor SwiftOnly the YoungPromotional single by Taylor SwiftReleasedJanuary 31, 2020 (2020-01-31)GenreSynth-popLength2:37LabelRepublicSongwriter(s) Taylor Swift Joel Little Producer(s) Taylor Swift Joel Little Lyric videoOnly the Young on YouTube Only the Young is a song by the American singer-songwriter Taylor Swift. It was released on January 31, 2020, through Republic Records, as a promotional single for Miss Americana, a 2020 Netflix documentary o...

 

约翰斯顿环礁

此條目可参照英語維基百科相應條目来扩充。 (2021年5月6日)若您熟悉来源语言和主题，请协助参考外语维基百科扩充条目。请勿直接提交机械翻译，也不要翻译不可靠、低品质内容。依版权协议，译文需在编辑摘要注明来源，或于讨论页顶部标记{{Translated page}}标签。 约翰斯顿环礁Kalama Atoll 美國本土外小島嶼 Johnston Atoll 旗幟颂歌：《星條旗》The Star-Spangled Banner約翰斯頓環礁�...

دابرة

هذه المقالة يتيمة إذ تصل إليها مقالات أخرى قليلة جدًا. فضلًا، ساعد بإضافة وصلة إليها في مقالات متعلقة بها. (أكتوبر 2023)Learn how and when to remove this message قطقاط شوكي الجناح أثناء الطيران له نتوءات جناح مرئية بوضوح على الحافة الأمامية للأجنحة. الدَّابِرة[1][2] أو المِهماز[3] أو �...

 

Patriarch Ponds

Park, pond and residential area in Moscow, Russia 55°45′50″N 37°35′32″E / 55.763883°N 37.592120°E / 55.763883; 37.592120 You can help expand this article with text translated from the corresponding article in Russian. (January 2017) Click [show] for important translation instructions. Machine translation, like DeepL or Google Translate, is a useful starting point for translations, but translators must revise errors as necessary and confirm that the tra...

 

Joe Lombardo

Governor of Nevada since 2023 This article is about the governor of Nevada. For the fictional character, see List of Third Watch characters § Firefighter Joe Lombardo. For the American mobster, see Joseph Lombardo. Joe LombardoLombardo in 202231st Governor of NevadaIncumbentAssumed office January 2, 2023LieutenantStavros AnthonyPreceded bySteve Sisolak17th Sheriff of Clark CountyIn officeJanuary 5, 2015 – January 2, 2023Preceded byDoug GillespieSucceeded byKevin McMahill ...

Virginia Film Festival

Annual film festival held in Charlottesville, USA This article has multiple issues. Please help improve it or discuss these issues on the talk page. (Learn how and when to remove these template messages) The topic of this article may not meet Wikipedia's notability guideline for events. Please help to demonstrate the notability of the topic by citing reliable secondary sources that are independent of the topic and provide significant coverage of it beyond a mere trivial mention. If notability...

 

SS-Verfugungstruppe

SS-VerfügungstruppeLambang SS yang dikenakan pada sebelah kanan helm SS-Verfügungstruppe.Aktif1934-1940Negara Jerman NaziAliansiAdolf HitlerCabang Waffen SSTipe unitInfanteri BermotorPeranSchnelltruppen (Pasukan Gesit)Jumlah personel1 DivisiMarkasBerlinTokohTokoh berjasaBrigadeführer Paul HausserOberführer Felix SteinerObergruppenfuhrer Sepp Dietrich SS-Verfugungstruppe (SS-VT) (bahasa Indonesia: Pasukan Disposisional SS) dibentuk pada tahun 1934 sebagai pasukan tempur NSDAP. Tah...

 

Wissenschaft des Judentums

Part of a series onJews and Judaism Etymology Who is a Jew? Religion God in Judaism (names) Principles of faith Mitzvot (613) Halakha Shabbat Holidays Prayer Tzedakah Land of Israel Brit Bar and bat mitzvah Marriage Bereavement Baal teshuva Philosophy Ethics Kabbalah Customs Rites Synagogue Rabbi Texts Tanakh Torah Nevi'im Ketuvim Talmud Mishnah Gemara Rabbinic Midrash Tosefta Targum Beit Yosef Mishneh Torah Tur Shulchan Aruch Zohar History General Timeline Land of Israel ...

Sungai Simpang Tangga

Sungai Simpang TanggaSungai Simpang TanggaLokasi mulut sungaiTampilkan peta KalimantanSungai Simpang Tangga (Indonesia)Tampilkan peta IndonesiaLokasiNegaraIndonesiaProvinsiKalimantan SelatanKotaBanjarmasinCiri-ciri fisikHulu sungai  - lokasiKalimantan Sungai Simpang Tangga adalah sungai yang mengalir di kota Banjarmasin, Kalimantan Selatan, Indonesia. Geografi Sungai ini mengalir di kecamatan Banjarmasin Utara.[1] Sungai Simpang Tangga memiliki panjang 723,371 m dengan ...

 

Takri (blok Unicode)

Artikel ini sebatang kara, artinya tidak ada artikel lain yang memiliki pranala balik ke halaman ini.Bantulah menambah pranala ke artikel ini dari artikel yang berhubungan atau coba peralatan pencari pranala.Tag ini diberikan pada April 2016. Takri (blok Unicode)JangkauanU+11680..U+116CF(80 titik kode)BidangSMPAksaraTakriAksara utamaChambealiDogriTerpakai66 titik kodeTak terpakai14 titik kode kosongRiwayat versi Unicode6.166 (+66) Catatan: [1] Takri adalah blok Unicode yang mengandung...