The top three runners in each of the initial ten heats automatically qualified for the second round. The next two fastest runners from across the heats also qualified for the second round. The top four runners in each of the four second round heats automatically qualified for the semi-final.
The gold medal was originally won by Marion Jones of the United States. However, on 5 October 2007, she admitted to having used performance-enhancing drugs prior to the 2000 Olympics. On 9 October she relinquished her medals to the United States Olympic Committee, and on 12 December the International Olympic Committee formally stripped her of her medals.
The IOC did not initially decide to regrade the results, as silver medalist Ekaterini Thanou had herself been subsequently involved in a doping scandal in the run-up to the 2004 Summer Olympics. After two years of deliberation, in late 2009 the IOC decided to upgrade Lawrence and Ottey to silver and bronze respectively, and leave Thanou as a silver medallist, with the gold medal withheld.
Records
Prior to this competition, the existing world and Olympic records were as follows: