A colony of Acromyrmex balzani consists of several subnests (i.e, polydomy), with one queen each. The underground nests contain 1–14 fungus chambers and can reach a depth of at least 160 cm (63.0 in). There are no waste chambers; the waste is deposited outside the nest.[2]
^ abcCaldato, Nádia; da Silva Camargo, Roberto; Forti, Luiz Carlos; de Andrade, Ana Paula Protti; Lopes, Juliane Floriano Santos (2016). "Nest architecture in polydomous grass-cutting ants (Acromyrmex balzani)". Journal of Natural History. 50 (25–26): 1561–1581. doi:10.1080/00222933.2016.1166529. hdl:11449/172806.