The enzyme 3′,5′-cyclic-GMP phosphodiesterase (EC 3.1.4.35) catalyzes the reaction

guanosine 3′,5′-cyclic phosphate + H₂O ${\displaystyle \rightleftharpoons }$ guanosine 5′-phosphate

This enzyme belongs to the family of hydrolases, specifically those acting on phosphoric diester bonds. The systematic name is 3′,5′-cyclic-GMP 5'-nucleotidohydrolase. Other names in common use include guanosine cyclic 3',5'-phosphate phosphodiesterase, cyclic GMP phosphodiesterase, cyclic 3′,5′-GMP phosphodiesterase, cyclic guanosine 3′,5′-monophosphate phosphodiesterase, cyclic guanosine 3′,5′-phosphate phosphodiesterase, cGMP phosphodiesterase, cGMP-PDE, and cyclic guanosine 3′,5′-phosphate phosphodiesterase.

As of late 2007, 5 structures have been solved for this class of enzymes, with PDB accession codes 1MC0, 2CHM, 2H40, 2H42, and 2H44.

• Marks F, Raab I (1974). "The second messenger system of mouse epidermis. IV. Cyclic AMP and cyclic GMP phosphodiesterase". Biochim. Biophys. Acta. 334: 368–377. doi:10.1016/0005-2744(74)90180-6.