The fixture list for the 2024 RFL League One was issued on 3 December 2023.[1] The regular season comprised 23 rounds to be followed by the play-offs. Each club had 20 fixtures with three bye weeks.
All times are UK local time (UTC±00:00 until 31 March 2024, UTC+01:00 thereafter).
Played between the losing team of the qualifying semi-final and the winning team of the elimination semi-final. The match was played at the ground of the team that finished higher during the regular season.
Played between the winning team of the qualifying semi-final and the winning team of the preliminary final. The match was played at the ground of the team that finished higher during the regular season.
Due to structure changes agreed before the season started, the play-off final winners did not automatically gain promotion and had to play a final match against the team that finishes 12th in the 2024 Championship. The winners of the match play in the Championship for 2025 and the losing team play in League One for 2025. Hunslet travelled to Swinton and won 22–20 to return to the Championship, from where they were relegated in 2013.