The opening match of the season happened on 25 July 2009 against Rot-Weiß Erfurt.[1] The match finished in a 0–0 draw.[2] Matchday two happened on 28 July 2009 against VfL Osnabrück.[1] Osnabrück won the match 1–0 with a goal from the penalty spot from Matthias Heidrich.[3] Matchday three happened on 7 August 2009 against Borussia Dortmund II.[1] Werder Bremen II won the match 1–0 with a goal from Torsten Oehrl.[4] Matchday four happened on 15 August 2009 against Holstein Kiel.[1] Holstein Kiel won the match 4–0 with three goals from Michael Holt and a goal from Fiete Sykora.[5] Matchday five happened on 22 August 2009 against Wacker Burghausen.[1] Wacker Burghausen won the match 4–3.[6]Onur Ayık, Torsten Oehrl, and Pascal Testroet scored for Werder Bremen II.[6] Wacker Burghausen got two goals from Christian Cappek and a goal each from Björn Hertl and Sven Kresin.[6] Matchday six happened on 30 August 2010 against Eintracht Braunschweig.[1] Werder Bremen II won the match 2–1.[7] Pascal Testroet scored two goals for Werder Bremen II and Dennis Kruppke scored for Eintracht Braunschweig.[7]
September and October
Matchday seven happened on 2 September 2009 against Jahn Regensburg.[1] Jahn Regensburg won the match 1–0 with a goal from Anton Shynder.[8] Matchday nine happened on 12 September 2009 against FC Ingolstadt 04.[1] Ingolstadt won the match 4–1.[9] Torsten Oehrl scored for Werder Bremen II.[9] Ingolstadt got two goals from Stefan Leitl, and a goal each from Moritz Hartmann and Robert Braber.[9] Matchday eight happened on 15 September 2009 against Kickers Offenbach.[1] Kickers Offenbach won the match 1–0 with a goal from David Ulm.[10] Matchday 10 happened on 19 September 2009 against SpVgg Unterhaching.[1] Werder Bremen II won the match 3–1.[11] Werder Bremen II got two goals from Onur Ayık and a goal from Nicolas Feldhahn.[11]Ömer Kanca scored for Unterhaching.[11]Felix Schiller was sent-off during the match.[11] Matchday 11 happened on 26 September 2009 against Carl Zeiss Jena.[1] The match finished in a 2–2 draw.[12] Pascal Testroet scored two goals for Werder Bremen II.[12]Orlando Smeekes and Salvatore Amirante scored for Carl Zeiss Jena.[12] Matchday 12 happened on 3 October 2009 against Dynamo Dresden.[1] Werder Bremen II won the match 2–0 with two goals from Torsten Oehrl.[13] Matchday 13 happened on 17 October 2009 against 1. FC Heidenheim.[1] Werder Bremen II won the match 2–1.[14] Pascal Testroet scored two goals for Werder Bremen II.[14]Dieter Jarosch scored for Heidenheim.[14] Matchday 14 happened on 24 October 2009 against Wuppertaler SV.[1] Wuppertal won the match 1–0 with a goal from Andrés Formento.[15] Matchday 15 happened on 30 October 2009 against VfB Stuttgart II.[1] Werder Bremen II won the match 1–0 with a goal from Onur Ayık.[16]
November and December
Matchday 16 happened on 7 November 2009 against Wehen Wiesbaden.[1] Werder Bremen II won the match 5–0 with two goals from Addy-Waku Menga and a goal each from Pascal Testroet, Stefan Ronneburg, and Onur Ayık.[17] Matchday 17 happened on 21 November 2009 against SV Sandhausen.[1] The match finished in a 2–2 draw.[18] Addy-Waku Menga scored two goals for Werder Bremen II.[18]Philipp Hosiner and Regis Dorn scored for Sandhausen.[18] Matchday 18 happened on 29 November 2009 against Bayern Munich II.[1] Bayern Munich II won the match 3–0 with two goals from Mehmet Ekici and a goal from Saër Sène.[19] Matchday 19 happened on 5 December 2009 against Erzgebirge Aue.[1] Erzgebirge Aue won the match 2–1.[20] Torsten Oehrl scored for Werder Bremen II.[20]Sebastian Glasner and Jan Hochscheidt scored for Erzgebirge Aue.[20]Alexander Hessel was sent-off during the match.[20] Matchday 20 happened on 12 December 2009 against Rot-Weiß Erfurt.[1] The match finished in a 1–1 draw.[21] Onur Ayık scored for Werder Bremen II and Petr Smíšek scored for Rot-Weiß Erfurt.[21]
March
Matchday 23 happened on 3 March 2010 against Holstein Kiel.[1] Werder Bremen II won the match 6–1.[22] Matchday 27 happened on 6 March 2010 against Kickers Offenbach.[1] Werder Bremen II got three goals from Onur Ayık and a goal each from Addy-Waku Menga, Kevin Artmann, and Lennart Thy.[22] Michael Holt scored for Holstein Kiel.[22] Kickers Offenbach won the match 4–0 with two goals from Steffen Haas and a goal each from Kai Hesse and Nils Pfingsten-Reddig.[23] Matchday 22 happened on 10 March 2010 against Borussia Dortmund II.[1] Werder Bremen II won the match 2–1.[24]Tobias Kempe scored two goals for Werder Bremen II.[24] Kempe's first goal was from the penalty spot.[24]Uwe Hünemeier scored from the penalty spot for Burussia Dortmund II.[24] Matchday 28 happened on 13 March 2010 against Ingolstadt.[1] The match finished in a 0–0 draw.[25] Matchday 21 happened on 16 March 2010 against Osnabrück.[1] Osnabrück won the match 1–0 with a goal from Alexander Dercho.[26] Matchday 21 happened on 21 March 2010 against Unterhaching.[1] Werder Bremen II won the match 1–0 with a goal from Nicolas Feldhahn.[27]Markus Schwabl was sent-off during the match.[27] Matchday 24 happened on 24 March 2010 against Wacker Burghausen.[1] The match finished in a 1–1 draw.[28] Lennart Thy scored for Werder Bremen II and Christian Holzer scored for Wacker Burghausen.[28] Matchday 30 happened on 28 March 2010 against Carl Zeiss Jena.[1] Carl Zeiss Jena won the match 2–1.[29] Kevin Artmann scored for Werder Bremen II.[29] Orlando Smeekes and Melvin Holwijn scored for Carl Zeiss Jena.[29]Márkó Futács was sent-off during the match.[29] Matchday 31 happened on 31 March 2010 against Dynamo Dresden.[1] Dynamo Dresden won the match 1–0 with a goal from Robert Koch.[30]
April and May
Matchday 32 happened on 3 April 2010 against Heidenheim.[1] The matchday finished in a 1–1 draw.[31] Onur Ayık scored for Werder Bremen II and Andreas Spann scored for Heidenheim.[31] Stefan Ronneburg was sent-off during the match.[31] Matchday 25 happened on 7 April 2010 against Eintracht Braunschweig.[1] Eintracht Braunschweig won the match 3–0 with goals from Deniz Doğan, Damir Vrančić, and Marco Calamita.[32] Matchday 33 happened on 10 April 2010 against Wuppertal.[1] The match finished in a 1–1 draw.[33]Sandro Wagner scored for Werder Bremen II and Nermin Čeliković scored for Wuppertal.[33] Matchday 34 happened on 13 April 2010 against Stuttgart II.[1] Stuttgart II won the match 3–0 with goals from Tobias Rathgeb, who scored from the penalty spot, Julian Schieber, and Sven Schipplock.[34] Matchday 35 happened on 17 April 2010 against Wehen Wiesbaden.[1] Wehen Wiesbaden won the match 2–0 with goals from Dominik Stroh-Engel and Aykut Öztürk.[35] Matchday 26 happened on 20 April 2010 against Jahn Regensburg.[1] Werder Bremen II won the match 4–1.[36] Werder Bremen II got three goals from Márkó Futács and a goal from Sandro Wagner.[36]Petr Stoilov scored for Jahn Regensburg.[36] Matchday 36 happened on 24 April 2010 against Sandhausen.[1] Werder Bremen II won the match 3–0 with goals from Timo Perthel, Sandro Wagner, and Onur Ayık.[37] Matchday 37 happened on 30 April 2010 against Bayern Munich II.[1] Bayern Munich II won the match 2–1.[38] Nicolas Feldhahn scored for Werder Bremen II.[38]Deniz Yılmaz and Saër Sène scored for Bayern Munich II.[38] Matchday 38 happened on 8 May 2010 against Erzgebirge Aue.[1] Werder Bremen II won the match 2–1.[39] Nicolas Feldhahn and Tobias Kempe scored for Werder Bremen II.[39] Sebastian Glasner scored for Erzgebirge Aue.[39]