Liga Deportiva Universitaria de Quito's 2000 season was the club's 70th year of existence, the 47th year in professional football, and the 40th in the top level of professional football in Ecuador.
Source: RSSSF Rules for classification: 1) points; 2) goal difference; 3) number of goals scored. Notes:
^El Nacional had three points deducted for fielding an ineligible player in Round 16 of the Second Stage.
^Since El Nacional qualified to the Liguilla Final twice, ESPOLI qualified to the last spot in the Liguilla Final as the best non-top-three finisher in the aggregate table.