2-Methylbutanoic acid, also known as 2-methylbutyric acid is a branched-chain alkylcarboxylic acid with the chemical formula CH3CH2CH(CH3)CO2H, classified as a short-chain fatty acid. It exists in two enantiomeric forms, (R)- and (S)-2-methylbutanoic acid. (R)-2-methylbutanoic acid occurs naturally in cocoa beans and (S)-2-methylbutanoic occurs in many fruits such as apples and apricots,[2][3] as well as in the scent of the orchid Luisia curtisii.[4]
History
2-Methylbutanoic acid is a minor constituent of Angelica archangelica and the perennial flowering plant valerian (Valeriana officinalis), where it co-occurs with valeric acid and isovaleric acid.[5] The dried root of this plant has been used medicinally since antiquity.[6] The chemical identity of all three compounds was first investigated in the 19th century by oxidation of the components of fusel alcohol, which includes the five-carbon amyl alcohols. Among the products isolated was a compound which gave a (+) rotation in polarised light, indicating it to be the (2S) isomer.[7]
Preparation
Racemic 2-methylbutanoic acid can readily be prepared by a Grignard reaction using 2-chlorobutane and carbon dioxide.[8]
It was the target of the first enantioselective synthesis in 1904 when the German chemist W. Marckwald heated ethyl(methyl)malonic acid with the chiral base brucine and obtained an optically active product mixture.[9] Either enantiomer of 2-methylbutanoic acid can now be obtained by asymmetric hydrogenation of tiglic acid using a ruthenium-BINAP catalyst.[10]
Reactions
The compound and its enantiomers react as typical carboxylic acids: they can form amide, ester, anhydride, and chloride derivatives.[11] The acid chloride is commonly used as the intermediate to obtain the others.
Uses
Racemic 2-methylbutanoic acid is a slightly volatile, colorless liquid with a pungent cheesy odor.[12] The smell differs significantly between the two enantiomeric forms. (S)-2-Methylbutyric acid has a pleasantly sweet, fruity odor[13] while (R)-2-methylbutanoic acid has a pervasive, cheesy, sweaty odor.[14] The main use of the materials, and their esters, is therefore as flavours and food additives.[2][15] The compounds' safety in this application was reviewed by an FAO and WHO panel, who concluded that there were no concerns at the likely levels of intake.[16]
Biology
Since 2-methylbutanoic acid and its esters are natural components of many foods, they are present in mammals including humans.[17][18]
^Mariaca, Raul G.; Imhof, Miroslava I.; Bosset, J. O. (2001). "Occurrence of volatile chiral compounds in dairy products, especially cheese - A review". European Food Research and Technology. 212 (3): 253–261. doi:10.1007/s002170000250. S2CID96864200.