Recruited from the 6th district, the regiment was organized at Indianapolis, Indiana, with a strength of 1,027 men[1] and mustered in on February 25, 1865.[1][2] It left Indiana for Nashville, Tennessee, on February 28. The regiment performed guard and garrison duty in the District of Middle Tennessee, Department of the Cumberland, to early September.[2] The regiment was mustered out on September 5, 1865.[1][2] During its service the regiment incurred thirty-six fatalities, and another seventy-five deserted.[1]