1000 series may refer to:

• Chichibu Railway 1000 series EMU, operated by the Chichibu Railway
• Choshi Electric Railway 1000 series EMU, operated by the Choshi Electric Railway
• Fukuoka Subway 1000 series EMU
• Hankyu 1000 series (1954) EMU, operated by Hankyu Corporation from 1954 to 1989
• Hankyu 1000 series EMU, operated by Hankyu Corporation since December 2013
• Hanshin 1000 series EMU, operated by the Hanshin Electric Railway
• JR Shikoku 1000 series DMU
• Keihan 1000 series EMU, operated by the Keihan Electric Railway
• Keikyu 1000 series EMU, operated by Keikyu
• Keikyu N1000 series EMU, operated by Keikyu
• Keio 1000 series (2nd generation) EMU, operated by Keio Corporation
• Meitetsu 1000 series EMU, operated by Meitetsu
• Nagano 1000 series EMU, operated by the Nagano Electric Railway
• Nagoya Municipal Subway N1000 series EMU
• Odakyu 1000 series EMU, operated by the Odakyu Electric Railway
• Osaka Monorail 1000 series EMU
• Sendai Subway 1000 series EMU
• Tokyo Metro 1000 series EMU
• Tokyo Monorail 1000 series EMU
• Tokyu 1000 series EMU
• Tōyō Rapid 1000 series EMU
• TX-1000 series EMU, operated by Tsukuba Express

• ATP World Tour Masters 1000, a series of nine tennis tournaments
• GeForce 1000 Series, a graphics processing units
• Washington Metro rolling stock § 1000-series

• Class 1000 Shinkansen

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