Znojmo District borders Austria in the south. The area is characterized by a slightly undulating and sparsely wooded landscape, with an above-average share of agricultural land and above-average temperatures. The territory extends into three geomorphological mesoregions: Jevišovice Uplands (most of the territory), Dyje–Svratka Valley (west) and Bobrava Highlands (small part in the northeast). The highest point of the district is a contour line in Zblovice with an elevation of 522 m (1,713 ft), the lowest point are the river beds of the Thaya and Jevišovka in Hrušovany nad Jevišovkou at 173 m (568 ft).
From the total district area of 1,590.4 km2 (614.1 sq mi), agricultural land occupies 1,069.3 km2 (412.9 sq mi), forests occupy 353.4 km2 (136.4 sq mi), and water area occupies 30.3 km2 (11.7 sq mi). Forests cover 22.2% of the district's area.[1]
The most important river is the Thaya, which flows across the southern part of the district. The Jevišovka flows through the central part and joins Thaya just behind the district border. The Rokytná flows through the northern part. The largest body of water is the Vranov Reservoir with an area of 762.5 ha (1,884 acres). Otherwise, there are not many bodies of water.
Along the Czech-Austrian border is the Podyjí National Park, the smallest Czech national park.
There are no motorways passing through the district. The most important roads are the I/38 (part of the European route E59) from Jihlava to Znojmo and the Czech-Austrian border, and the I/53 from Znojmo to Brno.