The film follows a selection of passengers on a long distance overnight flight on a silver Lockheed Constellation with Trans World Airlines, through a series of vignettes looking at the passengers and crew. Characters range from a precocious child star (intended to echo Shirley Temple) to businessmen and criminals.
An FBI man (Richard Travis) and a stewardess (Pamela Blake) solve a spy murder on the crowded airliner after a dead man is found in the toilet. All passengers are suspects. They discover the victim has been stabbed by a fountain pen containing Curare poison.
