He was born on 26 June 1659, the eldest surviving son[1] and heir of Sir Richard Brownlow, 2nd Baronet of Humby, Lincolnshire, by his wife Elizabeth Freke, a daughter of John Freke of Stretton in Dorset.
He was educated at Westminster School. In 1668 he succeeded his father as the 3rd baronet, of Humby, and in 1679 he inherited the estate of Belton, with others, from his childless great-uncle Sir John Brownlow, 1st Baronet. He built the present Belton House between 1685 and 1687, creating new gardens and lakes.[2]
In 1676 he married Alice Sherard (died 1721), a daughter of Richard Sherard of Lopethorp in Lincolnshire, by whom he had four (or five) daughters but no sons:
Sir John Brownlow committed suicide, aged only 38, in July 1697 after suffering from severe gout. As he died with no surviving sons, he was succeeded in his title and in most of his estates, including Belton, by his younger brother Sir William Brownlow, 4th Baronet, who received Belton House on condition that John's widow Alice Sherard should retain possession of it during her lifetime. As she outlived Sir William, it therefore passed on her death in 1721 to William's son John Brownlow, 1st Viscount Tyrconnel.