Spence was raised in Eltham and graduated from Eltham High School in 1988. She later graduating with a Bachelor of Laws and a Bachelor of Arts from the University of Tasmania in 2002.[3]
Prior to her election to Parliament, Spence worked as an electorate officer to various state and federal Members of Parliament, as operations manager for the Victorian branch of the Australian Labor Party, and as a volunteer solicitor at the Broadmeadows Legal Centre.[4][5]
Following her election, Spence served as Government Whip and later as Parliamentary Secretary for Public Transport and Parliamentary Secretary for Road Infrastructure. She has also served as the Chair of the Electoral Matters Committee.