RPE65

RPE65
Identifiers
AliasesRPE65, BCO3, LCA2, RP20, mrd12, sretinal pigment epithelium-specific protein 65kDa, retinal pigment epithelium specific protein 65, retinoid isomerohydrolase, p63, retinoid isomerohydrolase RPE65
External IDsOMIM: 180069; MGI: 98001; HomoloGene: 20108; GeneCards: RPE65; OMA:RPE65 - orthologs
Orthologs
SpeciesHumanMouse
Entrez

6121

19892

Ensembl

ENSG00000116745

ENSMUSG00000028174

UniProt

Q16518

Q91ZQ5

RefSeq (mRNA)

NM_000329

NM_029987

RefSeq (protein)

NP_000320

NP_084263

Location (UCSC)Chr 1: 68.43 – 68.45 MbChr 3: 159.3 – 159.33 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Retinal pigment epithelium-specific 65 kDa protein (also known as RPE65) is a retinoid isomerohydrolase enzyme of the vertebrate visual cycle.[5][6] RPE65 is expressed in the retinal pigment epithelium (RPE, a layer of epithelial cells that nourish the photoreceptor cells) and is responsible for the conversion of all-trans-retinyl esters to 11-cis-retinol during phototransduction.[7] 11-cis-retinol is then used in visual pigment regeneration in photoreceptor cells.[8][9] RPE65 belongs to the carotenoid oxygenase family of enzymes.[8]

Function

RPE65 is a critical enzyme in the vertebrate visual cycle found in the retinal pigmented epithelium. It is also found in rods and cones.[10] The photoisomerization of 11-cis-retinal to all-trans-retinal initiates the phototransduction pathway through which the brain detects light. All-trans-retinol is not photoactive and therefore must be reconverted to 11-cis-retinal before it can recombine with opsin to form an active visual pigment.[8][11] RPE65 reverses the photoisomerization by converting an all-trans-retinyl ester to 11-cis-retinol. Most commonly, the ester substrate is retinyl palmitate. The other enzymes of the visual cycle complete the reactions necessary to oxidize and esterify all-trans-retinol to a retinyl ester (RPE65's substrate) and to oxidize 11-cis-retinol to 11-cis-retinal (the required photoactive visual pigment component).[8][9]

The reaction completed by RPE65 in the retinoid cycle.

RPE65 is also referred to as retinol isomerase or retinoid isomerase, owing to past debates about the enzyme's substrate and whether it was involved in ester hydrolysis.[9]

Structure

RPE65 is a dimer of two symmetrical, enzymatically independent subunits. The active site of each subunit has a seven-bladed beta-propeller structure with four histidines that hold an iron(II) cofactor.[9][12] This structural motif is common across the studied members of the carotenoid oxygenase family of enzymes. RPE65 is strongly associated with the membrane of the smooth endoplasmic reticulum in RPE cells.[8]

Active site structure

The active site of each RPE65 active site contains an Fe(II) cofactor bound by four histidines (His180, His241, His313, and His527), each contributed by a separate blade on the beta-propeller structure. Three of the four histidines are coordinated to nearby glutamic acid residues (Glu148, Glu417, and Glu469), which are thought to help position the histidines to bind the iron cofactor in an octahedral geometry.[13] Phe103, Thr147, and Glu148 surround the active site where they help stabilize the carbocation intermediate and increase the stereoselectivity of RPE65 for 11-cis-retinol over 13-cis-retinol.[9]

The RPE65 iron(II) cofactor, showing its coordination with 4 histidine residues and 3 glutamic acid residues.

Reactants and products likely enter and leave the active site through a hydrophobic tunnel which is thought to open into the lipid membrane for direct lipid substrate absorption. A second, smaller tunnel also reaches the active site and may serve as a pathway for water, but is too narrow to transport the retinoid reactants and products.[9][13]

Membrane interactions

RPE65 is strongly associated with the membrane of the sER. sER is abnormally abundant in RPE cells due to their role in processing lipidic retinoids. Structural studies indicate that RPE65 is partially imbedded in the sER membrane via interactions between its hydrophobic face and the interior of the lipid membrane. This is supported by the need for detergent to solubilize RPE65. A major portion of RPE65's hydrophobic face, residues 109–126, forms an amphipathic alpha helix that likely contributes to the protein's membrane affinity. Additionally, Cys112 is palmitoylated in native RPE65, further supporting the theory that the hydrophobic face of RPE65 is imbedded in the membrane.[13]

The hydrophobic face contains the entrance to the large tunnel that leads to the enzyme's active site. The presence of this channel on the hydrophobic face combined with RPE65's demonstrated ability to absorb substrate direction from the lipid bilayer is consistent with RPE65 being partially embedded in the membrane.[8]

Conservation

RPE65 has been isolated from a wide range of vertebrates including zebra fish, chicken, mice, frogs, and humans.[8][14][15] Its structure is highly conserved between species, particularly in the beta-propeller and likely membrane bound regions. The amino acid sequences of human and bovine RPE65 differ by less than 1%.[13] The histidine residues of the beta-propeller structure and the bound iron(II) cofactor are 100% conserved across studied RPE65 orthologs and other members of the carotenoid oxygenase family.[9]

Soluble RPE65 (sRPE65)

Previously, it was proposed that RPE65 exists in two, interconverted forms: membrane bound mRPE65 and soluble sRPE65. This theory suggested that the reversible conversion of sRPE65 to mRPE65 by palmitoylation at Cys231, Cys329, and Cys330 played a role in regulating the retinoid cycle and endowing mRPE65 with its membrane affinity.[16] However, crystallographic studies of RPE65 have demonstrated that these residues are neither palmitoylated nor surface facing. New studies have also failed to confirm the presence of abundant soluble RPE65. Thus, this theory has been largely abandoned.[8][13]

Mechanism

The proposed RPE65 O-alkyl cleavage mechanism. The residues shown are, clockwise from top left - Phe103, Thr147, His313, His527, His180, His241, and Glu148.

RPE65 catalyzes the conversion of all-trans-retinyl ester to 11-cis-retinol through a proposed SN1 O-alkyl bond cleavage. RPE65's combination of an O-alkyl ester cleavage, geometric isomerization, and water addition is currently thought to be unique in biology. However, O-alkyl ester cleavage reactions with similarly stabilized carbocation intermediates are used by organic chemists.[9][17]

O-Alkyl cleavage

The O-alkyl cleavage of the ester bond, assisted by an Fe(II) cofactor, creates a carbocation intermediate that is stabilized by the conjugated polyene chain. The delocalization of the carbocation reduces the bond order of the polyene chain, thereby reducing the activation energy of the trans-to-cis isomerization. Phe103 and Thr178 additionally stabilize the isomerized carbocation and are thought to be responsible for the stereoselectivity of the enzyme. After isomerization, a nucleophilic attack by water at C15 restores the conjugation of the polyene chain and completes the ester bond cleavage.[9][13]

Alternate SN2 mechanism

Nearly all other biochemical ester hydrolysis reactions occur through an addition-elimination reaction at the acyl carbon. However, isotope labeling studies have demonstrated that the oxygen on the final 11-cis-retinol product of RPE65 originates from the solvent rather than the reacting ester, supporting the O-alkyl cleavage mechanism.[13] Another possible mechanism would begin with a nucleophilic attack at C11, but such an attack would rely on some nucleophile - most likely a cystine residue - to complete the isomerization portion of the reaction. Not only is the polyenyl ester probably not electron-poor enough to allow this reaction, but the active site region is lacking cystine residues to act as the nucleophile.[8][9]

Clinical significance

Mutations in this gene have been associated with Leber's congenital amaurosis type 2 (LCA2) and retinitis pigmentosa (RP).[6][18] RPE65 mutations are the most commonly detected mutations in LCA patients in Denmark.[19] The vast majority of RPE65 mutations in patients with LCA2 and RP occur in the beta-propeller regime and are believed to inhibit proper protein folding and iron cofactor binding. Particularly common propeller mutation sites are Tyr368 and His182. Substitution at Arg91 is also common and have been shown to impact RPE65 membrane interactions and substrate uptake.[13]

Though complete loss of function is associated with diseases such as LCA and RP, partial inhibition of RPE65 has been proposed as a treatment for age-related macular degeneration (AMD). All-trans-retinylamine (Ret-NH2) and emixustat have both been shown to competitively inhibit RPE65.[9] Emixustat is currently undergoing FDA phase 3 clinical trials as a therapy for AMD.[9][20]

Jean Bennett and Katherine A. High's work with the RPE65 mutation has reversed an inherited form of blindness. They received the first FDA approval of a gene therapy for a genetic disease, which is called Voretigene neparvovec.[citation needed]

See also

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000116745 – Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000028174 – Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Hamel CP, Tsilou E, Pfeffer BA, Hooks JJ, Detrick B, Redmond TM (Jul 1993). "Molecular cloning and expression of RPE65, a novel retinal pigment epithelium-specific microsomal protein that is post-transcriptionally regulated in vitro". The Journal of Biological Chemistry. 268 (21): 15751–7. doi:10.1016/S0021-9258(18)82319-5. PMID 8340400.
  6. ^ a b "Entrez Gene: RPE65 retinal pigment epithelium-specific protein 65kDa".
  7. ^ Wolf G (Mar 2005). "Function of the protein RPE65 in the visual cycle". Nutrition Reviews. 63 (3): 97–100. doi:10.1111/j.1753-4887.2005.tb00127.x. PMID 15825812.
  8. ^ a b c d e f g h i Kiser PD, Palczewski K (Sep 2010). "Membrane-binding and enzymatic properties of RPE65". Progress in Retinal and Eye Research. 29 (5): 428–42. doi:10.1016/j.preteyeres.2010.03.002. PMC 2903629. PMID 20304090.
  9. ^ a b c d e f g h i j k l Kiser PD, Zhang J, Badiee M, Li Q, Shi W, Sui X, Golczak M, Tochtrop GP, Palczewski K (Jun 2015). "Catalytic mechanism of a retinoid isomerase essential for vertebrate vision". Nature Chemical Biology. 11 (6): 409–15. doi:10.1038/nchembio.1799. PMC 4433804. PMID 25894083.
  10. ^ Tang, Peter H.; Buhusi, Mona C.; Ma, Jian-Xing; Crouch, Rosalie K. (2011-12-14). "RPE65 is present in human green/red cones and promotes photopigment regeneration in an in vitro cone cell model". The Journal of Neuroscience. 31 (50): 18618–18626. doi:10.1523/JNEUROSCI.4265-11.2011. ISSN 1529-2401. PMC 3297673. PMID 22171060.
  11. ^ Kiser PD, Golczak M, Palczewski K (Jan 2014). "Chemistry of the retinoid (visual) cycle". Chemical Reviews. 114 (1): 194–232. doi:10.1021/cr400107q. PMC 3858459. PMID 23905688.
  12. ^ Orban T, Jastrzebska B, Palczewski K (Apr 2014). "Structural approaches to understanding retinal proteins needed for vision". Current Opinion in Cell Biology. 27: 32–43. doi:10.1016/j.ceb.2013.11.001. PMC 3971393. PMID 24680428.
  13. ^ a b c d e f g h Kiser PD, Golczak M, Lodowski DT, Chance MR, Palczewski K (Oct 2009). "Crystal structure of native RPE65, the retinoid isomerase of the visual cycle". Proceedings of the National Academy of Sciences of the United States of America. 106 (41): 17325–30. doi:10.1073/pnas.0906600106. PMC 2765077. PMID 19805034.
  14. ^ Takahashi, Yusuke; Moiseyev, Gennadiy; Ma, Jian-xing (2014-09-26). "Identification of key residues determining isomerohydrolase activity of human RPE65". The Journal of Biological Chemistry. 289 (39): 26743–26751. doi:10.1074/jbc.M114.558619. ISSN 1083-351X. PMC 4175317. PMID 25112876.
  15. ^ Jin M, Li S, Moghrabi WN, Sun H, Travis GH (Aug 2005). "Rpe65 is the retinoid isomerase in bovine retinal pigment epithelium". Cell. 122 (3): 449–59. doi:10.1016/j.cell.2005.06.042. PMC 2748856. PMID 16096063.
  16. ^ Ma J, Zhang J, Othersen KL, Moiseyev G, Ablonczy Z, Redmond TM, Chen Y, Crouch RK (Jun 2001). "Expression, purification, and MALDI analysis of RPE65". Investigative Ophthalmology & Visual Science. 42 (7): 1429–35. PMID 11381042.
  17. ^ Redmond, T. Michael; Poliakov, Eugenia; Kuo, Stephanie; Chander, Preethi; Gentleman, Susan (2010-01-15). "RPE65, visual cycle retinol isomerase, is not inherently 11-cis-specific: support for a carbocation mechanism of retinol isomerization". The Journal of Biological Chemistry. 285 (3): 1919–1927. doi:10.1074/jbc.M109.027458. ISSN 1083-351X. PMC 2804350. PMID 19920137.
  18. ^ Bowne SJ, Humphries MM, Sullivan LS, Kenna PF, Tam LC, Kiang AS, Campbell M, Weinstock GM, Koboldt DC, Ding L, Fulton RS, Sodergren EJ, Allman D, Millington-Ward S, Palfi A, McKee A, Blanton SH, Slifer S, Konidari I, Farrar GJ, Daiger SP, Humphries P (Oct 2011). "A dominant mutation in RPE65 identified by whole-exome sequencing causes retinitis pigmentosa with choroidal involvement". European Journal of Human Genetics. 19 (10): 1074–81. doi:10.1038/ejhg.2011.86. PMC 3190249. PMID 21654732.
  19. ^ Astuti GD, Bertelsen M, Preising MN, Ajmal M, Lorenz B, Faradz SM, Qamar R, Collin RW, Rosenberg T, Cremers FP (Dec 2015). "Comprehensive genotyping reveals RPE65 as the most frequently mutated gene in Leber congenital amaurosis in Denmark". European Journal of Human Genetics. 24 (7): 1071–1079. doi:10.1038/ejhg.2015.241. PMC 5070892. PMID 26626312.
  20. ^ "Acucela - Retinal Diseases". acucela.com. Retrieved 2016-03-01.

Further reading

Protein Structure and Function
Clinical and Genetic Studies

Read other articles:

Iride (sommergibile)

IrideDescrizione generale TipoSommergibile di piccola crociera ClassePerla ProprietàRegia Marina CantiereOTO, La Spezia Impostazione3 settembre 1935 Varo30 luglio 1936 Entrata in servizio6 novembre 1936 Destino finaleaffondato da aerosiluranti il 22 agosto 1940 Caratteristiche generaliDislocamento in immersione856,397 t Dislocamento in emersione697,254 t Lunghezzafuori tutto 60,18 m Larghezza6,45 m Pescaggio4,66 m Profondità operativa80 m Propulsione2 motori di...

 

Takinoue, Hokkaido

Takinoue 滝上町KotaprajaBalai Kota Takinoue BenderaEmblemLokasi Takinoue di Hokkaido (Subprefektur Okhotsk)TakinoueLokasi di JepangKoordinat: 44°12′N 143°5′E / 44.200°N 143.083°E / 44.200; 143.083Koordinat: 44°12′N 143°5′E / 44.200°N 143.083°E / 44.200; 143.083NegaraJepangWilayahHokkaidoPrefektur Hokkaido (Subprefektur Okhotsk)DistrikMonbetsuPemerintahan • WalikotaNaohiro KiyoharaLuas • Total766,89...

 

Imam Sopingi

Imam Sopingi Kepala Badan Intelijen Negara Daerah Maluku UtaraMasa jabatan26 April 2021 – 27 Juni 2022 PendahuluBrigjen TNI Dudy FristiyantoPenggantiBrigjen TNI Benny BintoroWakil Komandan Lantamal IX/AmbonMasa jabatan1 Mei 2017 – 28 Mei 2018 PendahuluKolonel (Mar) Joko SupriyantoPenggantiKolonel (Mar) SupriyonoKomandan Komando Latih MarinirMasa jabatan17 Juni 2015 – 27 Febuari 2017 PendahuluKolonel (Mar) Budi PurnamaPenggantiKolonel (Mar) Wurjanto Informasi p...

Kabinet Adil

Kabinet AdilKabinet Pemerintahan Pasundan ke-1Dibentuk08 Mei 1948 (1948-05-08)Diselesaikan19 Desember 1948 (1948-12-19)Struktur pemerintahanKepala negaraWiranatakusumahKepala pemerintahanAdil PuradiredjaJumlah menteri8SejarahFormasi berikutnya10 Januari 1949PenggantiKabinet Djumhana I Kabinet Adil adalah kabinet pertama yang dibentuk oleh Negara Pasundan usai pembentukan negara tersebut pada 28 April 1948. Kabinet tersebut terdiri dari 8 menteri dan 1 pejabat. Masa jabatannya berlan...

 

Carlos Aguiar Retes

Mexican cardinal of the Catholic Church In this Spanish name, the first or paternal surname is Aguiar and the second or maternal family name is Retes. His EminenceCarlos Aguiar RetesCardinalArchbishop of Mexico CityChurchRoman Catholic ChurchArchdioceseMexico CitySeeMexico CityAppointed7 December 2017Installed5 February 2018PredecessorNorberto Rivera CarreraOther post(s)Cardinal-Priest of Santi Fabriano e Venanzio a Villa Fiorelli (since 2016)OrdersOrdination22 April 1973Consecration2...

 

Oliver Bernard

English poet and translator For his father, the English architect and designer, see Oliver Percy Bernard. Oliver Bernard (6 December 1925 – 1 June 2013)[1] was an English poet and translator. He is perhaps best known for translating Arthur Rimbaud into English as part of the Penguin Classics collection. Bernard's former house, Poets' Corner, in Kenninghall, where a memorial plaque has been erected. Bernard was born in London, to the English architect Oliver Percy Bernard OBE and the...

Batas divergen

Jembatan di sepanjang lembah celah Álfagjá di Islandia. Lembah celah tersebut merupakan bagian dari batas divergen antara lempeng Eurasia dan Amerika Utara. Dalam tektonika lempeng, batas divergen atau batas lempeng divergen merupakan ciri linear yang ada di antara dua lempeng tektonik yang saling menjauh. Batas divergen di benua biasanya menghasilkan celah yang kemudian menjadi lembah celah. Sebagian besar batas lempeng divergen yang aktif ada di lempeng samudra dan menghasilkan punggung t...

 

Turks in the Tang military

Overview of the use of Turkic troops in the Tang dynasty military History of the Turkic peoples pre–14th centuryCourt of Seljuk ruler Tughril III, circa 1200 CE. Turkic peoples Oghuz Turks Karluks Kipchaks Onogurs Kumyks Yakuts Dolgans Krymchaks Sabir people Crimean Karaites Turkic Languages Turkish Azerbaijani Uzbek Kazakh Uyghur Turkmen Tatar Kyrgyz Bashkir Chuvash Qasgqai Karakalpak Sakha Kumyk Karachay-Balkar Tuvan Gagauz Karaim Krymchak Turkic Mythology Belief system: Tengrism and Sham...

 

Galvanic corrosion

Electrochemical process Corrosion of an iron nail wrapped in bright copper wire, showing cathodic protection of copper; a ferroxyl indicator solution shows colored chemical indications of two types of ions diffusing through a moist agar medium Galvanic corrosion (also called bimetallic corrosion or dissimilar metal corrosion) is an electrochemical process in which one metal corrodes preferentially when it is in electrical contact with another, in the presence of an electrolyte. A similar galv...

List of U.S. Highways in Maryland

United States Numbered Highways of the Maryland highway system Standard route signs in MarylandHighway namesUS HighwaysU.S. Route X (US X)Special Routes: U.S. Route X Alternate (US X Alt.); U.S. Route X Business (US X Bus.); U.S. Route X Scenic (US X Scenic); U.S. Route X Truck (US X Truck) System links Maryland highway system Interstate US State Scenic Byways The following is a list of U.S. Highways in Maryland. There are currently 14 U.S. Highways that exist entirely or partially in t...

 

Mostafa Madbouly

Prime Minister of Egypt (2018–present) Mostafa Madboulyمصطفى مدبوليMadbouly in 202054th Prime Minister of EgyptIncumbentAssumed office 14 June 2018Acting: 7 June 2018 – 14 June 2018PresidentAbdel Fattah el-SisiPreceded bySherif IsmailMinister of Housing, Utilities & Urban CommunitiesIn office1 March 2014 – 14 February 2019Prime MinisterIbrahim MahlabSherif IsmailHimselfPreceded byIbrahim MahlabSucceeded byAssem el Gazzar Personal detailsBorn (1966-04-28) 28 A...

 

Arsitek

Arsitek di depan papan gambarnya, 1893. Arsitek adalah seorang ahli di bidang ilmu arsitektur, ahli rancang bangun atau ahli lingkungan binaan. Istilah arsitek sering kali diartikan secara sempit sebagai seorang perancang bangunan, adalah orang yang terlibat dalam perencanaan, merancang, dan mengawasi konstruksi bangunan, yang perannya untuk memandu keputusan yang memengaruhi aspek bangunan tersebut dalam sisi astetika, budaya, atau masalah sosial. Definisi tersebut kuranglah tepat karena lin...

Zuiweng Pavilion

Building in China Zuiweng Pavilion The Zuiweng Pavilion (Chinese: 醉翁亭; pinyin: Zùiwēng Tíng; lit. 'Old Toper's Pavilion') is a pavilion lying to the south east of Chuzhou City, Anhui Province, People's Republic of China. Located in The Northern Song Dynasty whilst the structure that exists today dates to the Qing Dynasty (1644–1911). The pavilion takes its name from the Northern Song poet Ouyang Xiu, who called himself the Old Toper and wrote a poem entitled Zui...

 

Lingua yiddish

YiddishייִדישPronuncia[ˈ(j)ɪdɪʃ] Parlato in Stati Uniti Svezia Israele Ucraina Germania Bielorussia Russia Polonia Canada Romania Moldavia Argentina Regioni Russia Oblast' autonoma ebraica LocutoriTotale3 milioni (1991) Altre informazioniScritturaalfabeto ebraico TassonomiaFilogenesiLingue indoeuropee Lingue germaniche  Lingue germaniche occidentali   Lingue erminoniche   &#...

 

Usmate Velate

Peta wilayah Komune Usmate Velate (merah) di Provinsi Monza dan Brianza (emas), Lombardia, Italia. Usmate Velate komune di Italia Tempat Negara berdaulatItaliaDaerah di ItaliaLombardyProvinsi di ItaliaProvinsi Monza dan Brianza NegaraItalia PendudukTotal10.547  (2023 )GeografiLuas wilayah9,75 km² [convert: unit tak dikenal]Ketinggian221 m Berbatasan denganArcore Camparada Casatenovo (en) Lomagna (en) Vimercate Carnate Informasi tambahanKode pos20865 Zona waktuUTC+1 UTC+2 Kode telep...

Comet Rendezvous, Sample Acquisition, Investigation, and Return

COmet Rendezvous, Sample Acquisition, Investigation, and Return (CORSAIR) is a concept mission to return comet nucleus samples to Earth for detailed analysis. The mission concept was submitted in May 2017 by a team from NASA's Goddard Space Flight Center in response to the New Frontiers program call for mission 4, but did not pass the initial down selection. As a comet sample return mission was not ultimately selected for mission 4 (Dragonfly, a Titan probe was selected), the CORSAIR team may...

 

Patrick Borg

Pour les articles homonymes, voir Borg. Patrick Borg Patrick Borg à la Japan Con Brussels Manga, en février 2023. Données clés Naissance 6 août 1957 (66 ans)Paris (France) Nationalité Française Profession Acteur Séries notables (Doublage ) Dragon Ball ZDragon Ball Z KaiDragon Ball SuperScooby-Doo : Mystères associés Buffy contre les vampires modifier Patrick Borg, né le 6 août 1957 à Paris, est un comédien français. Spécialisé dans le doublage, il est connu pour ê...

 

Masfout

Place in Ajman, United Arab EmiratesMasfoutMasfoutCoordinates: 24°48′54″N 56°5′52″E / 24.81500°N 56.09778°E / 24.81500; 56.09778CountryUnited Arab EmiratesEmirateAjmanArea • Land86.59 km2 (33.43 sq mi)Elevation387 m (1,269 ft)Population (2017) • Total8,988 • Density103.8/km2 (269/sq mi) Masfout is a village that forms part of the eponymous exclave of Masfout in Ajman, one of the seven em...

Someone like You (Adele song)

2011 single by Adele Someone like YouSingle by Adelefrom the album 21 Released24 January 2011 (2011-01-24)Recorded2010GenreSoulpopLength4:45LabelXLColumbiaSongwriter(s)Adele AdkinsDan WilsonProducer(s)Adele AdkinsDan WilsonAdele singles chronology Rolling in the Deep (2010) Someone like You (2011) Set Fire to the Rain (2011) Music videoSomeone like You on YouTube Someone like You is a song recorded by English singer-songwriter Adele. She and Dan Wilson wrote and produced the tr...

 

Kerens, Texas

City in Texas, United StatesKerens, TexasCityDowntown KerensMotto: Birthplace Of Big Tex[1]Location of Kerens, TexasCoordinates: 32°7′50″N 96°13′45″W / 32.13056°N 96.22917°W / 32.13056; -96.22917CountryUnited StatesStateTexasCountyNavarroArea[2] • Total2.50 sq mi (6.48 km2) • Land2.50 sq mi (6.48 km2) • Water0.00 sq mi (0.01 km2)Elevation371 ft (113&#...