Family of transport proteins
The NhaC family (TC# 2.A.35 ) belongs to the Ion Transporter (IT) Superfamily .[ 1] Transporter Classification Database .
Two members of the NhaC family have been functionally characterized. One is believed to be a Na+ :H+ antiporter;[ 2] + :lactate·Na+ antiporter.[ 3] Vibrio cholerae ,Haemophilus influenzae Bacillus subtilis .E. coli Pyrococcus Gram-negative bacteria and Gram-positive bacteria as well as archaea. NhaC of B. firmus is 462 amino acyl residues long and possesses 12 putative transmembrane α-helical segments. MleN of B. subtilis (468 aas; TC# 2.A.35.1.2 ) also exhibits 12 putative TMSs.
The transport reaction catalyzed by NhaC is probably:
Na+ (in) + nH+ (out) ⇌ Na+ (out) + nH+ (in). (n > 1)
That catalyzed by MleN is probably:
Malate (out) + H+ (out) + Lactate (in) + Na+ (in) ⇌ Malate (in) + H+ (in) + Lactate (out) + Na+ (out)
See also
Further reading
Ivey, D. M.; Guffanti, A. A.; Bossewitch, J. S.; Padan, E.; Krulwich, T. A. (1991-12-05). "Molecular cloning and sequencing of a gene from alkaliphilic Bacillus firmus OF4 that functionally complements an Escherichia coli strain carrying a deletion in the nhaA Na+/H+ antiporter gene" . The Journal of Biological Chemistry 266 (34): 23483–23489. ISSN 0021-9258 . PMID 1660475 .
Liew, Chong Wai; Illias, Rosli Md; Mahadi, Nor Muhammad; Najimudin, Nazalan (2007-11-01)."Expression of the Na+/H+ antiporter gene (g1-nhaC) of alkaliphilic Bacillus sp. G1 in Escherichia coli" . FEMS Microbiology Letters 276 (1): 114–122. doi :10.1111/j.1574-6968.2007.00925.x . ISSN 0378-1097 . PMID 17937670 .
Panina, Ekaterina M.; Vitreschak, Alexey G.; Mironov, Andrey A.; Gelfand, Mikhail S. (2003-05-28)."Regulation of biosynthesis and transport of aromatic amino acids in low-GC Gram-positive bacteria" . FEMS Microbiology Letters 222 (2): 211–220. ISSN 0378-1097 .PMID 12770710 .
Ravcheev, Dmitry A.; Best, Aaron A.; Tintle, Nathan; Dejongh, Matthew; Osterman, Andrei L.; Novichkov, Pavel S.; Rodionov, Dmitry A. (2011-07-01)."Inference of the transcriptional regulatory network in Staphylococcus aureus by integration of experimental and genomics-based evidence" . Journal of Bacteriology 193 (13): 3228–3240. doi :10.1128/JB.00350-11 . ISSN 1098-5530 . PMC 3133287 . PMID 21531804 .
Rodionov, Dmitry A.; Hebbeln, Peter; Eudes, Aymerick; ter Beek, Josy; Rodionova, Irina A.; Erkens, Guus B.; Slotboom, Dirk J.; Gelfand, Mikhail S.; Osterman, Andrei L. (2009-01-01). "A novel class of modular transporters for vitamins in prokaryotes" . Journal of Bacteriology 191 (1): 42–51. doi :10.1128/JB.01208-08 .ISSN 1098-5530 . PMC 2612444 . PMID 18931129 .
References
^ Prakash, Shraddha; Cooper, Garret; Singhi, Soumya; Saier, Milton H. (2003-12-03). "The ion transporter superfamily" . Biochimica et Biophysica Acta (BBA) - Biomembranes . 1618 (1): 79– 92. doi :10.1016/j.bbamem.2003.10.010 ISSN 0006-3002 . PMID 14643936 . ^ Ito, M.; Guffanti, A. A.; Zemsky, J.; Ivey, D. M.; Krulwich, T. A. (1997-06-01). "Role of the nhaC-encoded Na+/H+ antiporter of alkaliphilic Bacillus firmus OF4" . Journal of Bacteriology . 179 (12): 3851– 3857. doi :10.1128/jb.179.12.3851-3857.1997 . ISSN 0021-9193 . PMC 179192 PMID 9190799 . ^ Wei, Y.; Guffanti, A. A.; Ito, M.; Krulwich, T. A. (2000-09-29). "Bacillus subtilis YqkI is a novel malic/Na+-lactate antiporter that enhances growth on malate at low protonmotive force" . The Journal of Biological Chemistry . 275 (39): 30287– 30292. doi :10.1074/jbc.M001112200 ISSN 0021-9258 . PMID 10903309 .
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