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MID2
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	2DJA, 2DMK
Identifiers
Aliases	MID2, FXY2, MRX101, RNF60, TRIM1, midline 2, XLID101
External IDs	OMIM: 300204; MGI: 1344333; HomoloGene: 8028; GeneCards: MID2; OMA:MID2 - orthologs
Gene location (Human)
Chr.	X chromosome (human)
End	107,931,637 bp
Gene location (Mouse)
Chr.	X chromosome (mouse)
End	139,668,464 bp
RNA expression pattern
	Top expressed in
	tibialis anterior muscle; ; islet of Langerhans; ; cartilage tissue; ; muscle of thigh; ; smooth muscle tissue; ; gastrocnemius muscle; ; skin of thigh; ; pancreatic ductal cell; ; popliteal artery; ; tibial arteries;
	Top expressed in
	lateral nasal prominence; ; medial nasal prominence; ; left lung lobe; ; supraoptic nucleus; ; female urethra; ; superior frontal gyrus; ; dentate gyrus of hippocampal formation granule cell; ; genital tubercle; ; ascending aorta; ; cerebellar cortex;
	More reference expression data
	More reference expression data
Gene ontology
Molecular function	protein homodimerization activity; microtubule binding; zinc ion binding; metal ion binding; phosphoprotein binding; protein binding; protein heterodimerization activity; identical protein binding; transferase activity;
Cellular component	cytoplasm; intracellular anatomical structure; microtubule; extracellular exosome; cytoskeleton;
Biological process	positive regulation of autophagy; positive regulation of DNA-binding transcription factor activity; protein localization to microtubule; positive regulation of NF-kappaB transcription factor activity; protein ubiquitination; positive regulation of I-kappaB kinase/NF-kappaB signaling; innate immune response; negative regulation of viral transcription; negative regulation of viral entry into host cell;
	Sources:Amigo / QuickGO
Orthologs
	11043
	23947
	ENSG00000080561
	ENSMUSG00000000266
	Q9UJV3
	Q9QUS6
	NM_012216; NM_052817; NM_001382751; NM_001382752
	NM_011845; NM_001358366; NM_001358367
	NP_036348; NP_438112; NP_001369680; NP_001369681
	NP_035975; NP_001345295; NP_001345296; NP_001390295
	Wikidata
View/Edit Human	View/Edit Mouse

Midline-2 is a protein that in humans is encoded by the MID2 gene.^[5]^[6]

Function

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Its function has not been identified. Alternate splicing of this gene results in two transcript variants encoding different isoforms.^[6]

Recent reports indicate the involvement of MID2 in cytokinesis ^[7]^[8].MID2 (TRIM1) ubiquitinates Sperm-associated antigen 5 (Astrin) on K409, further promoting its degradation and proper cytokinesis.^[8] In contrary, depletion of MID2 (TRIM1) stabilizes Sperm-associated antigen 5 (Astrin) whose inappropriate accumulation at the midbody triggers cytokinetic arrest, multinucleated cells, and cell death.^[7]^[8]

Interactions

MID2 has been shown to interact with MID1.^[9]^[10]

MID2 (TRIM1) interacts with Leucine-rich repeat kinase 2 (LRRK2), which is often subject to missense mutations in familial Parkinson's disease (PD).^[11] MID2 (TRIM1) specifically binds to the flexible regulatory loop of LRRK2_853–981.^[11] MID2 (TRIM1) recruits LRRK2 to the microtubule cytoskeleton where MID2 (TRIM1) ubiquitinates LRRK2 targeting it for proteasomal degradation.^[11]