On Monday, July 18, 2005, Canal de las Estrellas started broadcasting La esposa virgen weekdays at 9:00pm, replacing La madrastra. The last episode was broadcast on Friday, October 21, 2005 with Alborada replacing it on Monday, October 24, 2005.
Shortly before his death, general Francisco Ortiz marries Virginia and appoints her as his sole heiress. When Virginia and her younger brother Diego arrive to the town of San Francisco de los Arenales to bury the general, accompanied with the nephew of captain Fernando Ortiz, who despises Virginia, believing that she is a gold-digger who married her uncle for his fortune.
The only person that receives Virginia politely in the town is the municipal President, Dr. José Guadalupe Cruz, a good and honest man whose wife, Blanca, suffers from a terminal disease.
Virginia is surprised to see the ranch of the general is abandoned, without knowing that this is work of the mother of Fernando, Aurelia, who has allied with the administrator, Cristóbal, to steal the fortune that Virginia has inherited.
Aurelia hates Virginia and will try with all means that Virginia leaves the ranch because she knows if she does so, she will lose her inheritance, but if she doesn't, Aurelia is ready to resort to violence. Her hatred is even greater when she realizes that Fernando is madly love with Virginia, although Virginia only sees him as a good friend.
When Virginia meets Blanca, a sincere friendship begins between them. José Guadalupe ignores that Blanca is aware of his infidelity with a selfish and ambitious woman named Olga.
Knowing that he has little time of life, Blanca fears for the happiness of her husband and her daughter Marisol if he were to marry Olga.
Unintentionally, a passionate love is born between Virginia and José Guadalupe. Both refuse to accept it and treat each other with hostility, because they know that giving themselves to that love would be betraying the trust of Blanca.
After a marriage which was never consummated, Virginia will now have to combat hatred and the ambition of Aurelia and Cristóbal, while trying uselessly to ignore the love that she feels for José Guadalupe.
