John Robert Edward Lee was born into slavery to John and Mary (Mayes) Lee in Seguin, Texas. He attended Bishop College, starting in 1883 with preparatory classes and graduating with honors in 1889, the third person to graduate with an A.B. from Bishop. He spent the next two years as the principal of a two-teacher school in Palestine, Texas. In 1891, he returned to Bishop to spend the next decade there as a faculty member, serving as Dean of Men and Professor of history, mathematics, and Latin. In 1895, he married Ardelia Wilson and they would have seven children.[1][2][4]
Following a recommendation from Jackson Davis, Lee became President of Florida Agricultural and Mechanical University in 1924. Lee obtained greater funding for the university from the Florida Board of Control, the General Education Board, and the Rosenwald Fund and accreditation for the university from the Southern Association of Colleges and Secondary Schools. He was able to obtain greater salaries for teachers and administrators despite the statement of Florida Governor Fred P. Cone that "no Negro was worth $4000 a year".[2] By the end of Lee's tenure, "FAMU had constructed 48 buildings, accumulated 396 acres of land, and had 812 students and 122 staff members."[3]