At the entrance of Witness Tree Junction, a trailhead along the Gainesville-Hawthorne State Trail, a trio of live oak trees sport carvings demanding notice of Colonel Daniel Newnan and his 100 soldiers' march to capture runaway Black Seminoles during the Patriot War of 1812.[3] These three trees are also marked by N.R. Gruelle, a surveyor for the Florida Southern Railway (originally the Gainesville, Ocala and Charlotte Harbor Railroad Company), to delineate one end of railroad property.[3]
The Boulware Springs trailhead is located at Boulware Springs Nature Park, 3300 S.E. 15th St, Gainesville, and is open from 8 a.m. to 6 p.m. November through April and from 8 a.m. to 8 p.m. May through October.
Other trailheads with parking include (1) intersection of county roads 234 and 2082, Rochelle (at Prairie Creek Preserve); (2) 7902 S.E. 200th Drive, off County Road 2082, west of Hawthorne; and (3) 2182 S.E. 71st Avenue, Hawthorne.[4]
The Paynes Prairie portion of the trail is open from 8 a.m. until sunset, 365 days a year.