A follow-up to the 1956 episode "Our Unsung Villains", this updated version was used to showcase Madame Medusa, the newest villainess in the then upcoming animated film, The Rescuers. Hans Conried reprises his role as the Magic Mirror from "Our Unsung Villains" and other television specials to host the program. The Magic Mirror explains the importance of villains and introduces clips of various Disney villains while also providing biased, yet humorous, commentary. Most of the villains received a good-sized clip from their respective films, although some were featured in very brief 30-second segments. One sidenote is that the music featured in Madame Medusa's segment was not used in The Rescuers.
"Disney's Greatest Villains" would be shown again in reruns, the last time being on the Vault Disney block of programming on the Disney Channel. In 1983, portions of "Disney's Greatest Villains" were incorporated into the 90-minute special "A Disney Halloween".
