The synagogue was built in 1836 and was used by Jewish community in Prostějov to 1939. In 1951–1963 and 2004–2018, the building was used by orthodox Christian community. In some of the intervening years, the building was used as an art gallery.
The building was transferred to private ownership. In March 2024 it was reported that the former synagogue was for sale for approximately € 217,000.[3][4]