Running unobtrusively on the outside, Anna Cockrell pushed out to an early lead down the backstretch. Through the final turn Zurian Hechaverría asserted herself, pulling ahead, with Sage Watson the closes competitor over a stride behind. Watson pulled even as they cleared the 9th hurdle, but upon landing Watson exploded ahead as Hechaverría was swimming in mud. Rushell Clayton was the closest chaser as they ran for home, but she wasn't making up any ground on Watson's lead. Watson went on to an easy victory in 55.16. Cockrell made a late rush to nip Clayton at the line for silver.
Records
Prior to this competition, the existing world and Pan American Games records were as follows: