The 2023 Cali Open was a professional tennis tournament played on clay courts. It was the second edition of the tournament which was part of the 2023 ATP Challenger Tour. It took place in Cali, Colombia between 19 and 24 June 2023.
The following players received wildcards into the singles main draw:
The following players received entry into the singles main draw using protected rankings:
The following players received entry into the singles main draw as alternates:
The following players received entry from the qualifying draw:
