Source: 2020 USL League One standings Rules for classification: 1) points; 2) total wins; 3) total goal differential; 4) total goals scored; 5) points earned against top four Conference finishers; 6) disciplinary points; 7) coin toss or drawing of lots Notes:
^ abcProfessional teams that are owned by teams playing in a higher division are ineligible for the U.S. Open Cup.