The 2010 Regional League Division 2 (also known as the AIS Regional League Division 2 for sponsorship reasons) was contested by the five regional league winners and runners up of the 3rd level championships of Thailand. The two best 3rd placed teams from the regional leagues also take part
Twelve teams were split into two groups of A & B, with the top two teams from group A & B gaining promotion to the Thai 1st Division for the 2011 campaign, along with this, the two group winners would play off to determine the overall champions.
The teams finishing in 3rd and 4th position would enter another round of playoffs with the bottom four sides of the Thai Division 1 League to determine if they would play in the 1st Division for the 2011 campaign.
Locations of the Regional League Division 2 All Teams in 2010 2010 Regional League Division 2 Bangkok & field Region 2010 Regional League Division 2 Central & Eastern Region 2010 Regional League Division 2 Northern Region 2010 Regional League Division 2 North Eastern Region 2010 Regional League Division 2 Southern Region