The Women's 500m Time Trial was one of the 6 women's events at the 2005 UCI Track Cycling World Championships, held in Los Angeles, United States.

18 Cyclists from 14 countries were due to participate in the contest, Christin Muche of Germany did not start. The Final was held on March 24 at 19:35.

World Record
WR	33.952	Anna Meares (AUS)	Athens	August 20, 2004

Rank[1]	Name	250m	Time	Speed (km/h)
			250-500
	Natallia Tsylinskaya (BLR)	19.453 (2)	34.738	51.816
			15.285 (5)
	Anna Meares (AUS)	19.484 (3)	34.752	51.795
			15.268 (3)
	Yvonne Hijgenaar (NED)	19.703 (4)	34.928	51.534
			15.225 (2)
4	Willy Kanis (NED)	19.233 (1)	35.056	51.346
			15.823 (12)
5	Victoria Pendleton (GBR)	19.815 (5)	35.088	51.299
			15.273 (4)
6	Tamilla Abassova (RUS)	19.954 (8)	35.109	51.268
			15.155 (1)
7	Lori-Ann Muenzer (CAN)	19.824 (6)	35.217	51.111
			15.393 (7)
8	Simona Krupeckaitė (LTU)	19.941 (7)	35.376	50.881
			15.435 (8)
9	Elisa Frisoni (ITA)	20.085 (11)	35.395	50.854
			15.310 (6)
10	Céline Nivert (FRA)	19.996 (9)	35.628	50.522
			15.632 (10)
11	Clara Sanchez (FRA)	20.186 (15)	35.730	50.377
			15.544 (9)
12	Fang Tian (CHN)	20.183 (14)	35.974	50.036
			15.791 (11)
13	Rebecca Conzelman (USA)	20.057 (10)	36.074	49.897
			16.017 (14)
14	Susann Panzer (GER)	20.129 (12)	36.122	49.831
			15.993 (13)
15	Nancy Contreras (MEX)	20.159 (13)	36.343	49.528
			16.184 (15)
16	Magdalena Sara (POL)	20.727 (16)	36.983	48.671
			16.256 (16)
17	Alena Prudnikova (RUS)	20.908 (17)	37.497	48.003
			16.589 (17)
	Christin Muche (GER)	20.353 (16)	DNS