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The 2000 Peruvian Segunda División, the second division of Peruvian football (soccer), was played by 13 teams. The tournament winner, Deportivo Aviación was promoted to the Playoff. The tournament was played on a home-and-away round-robin basis.

Sporting Cristal B can´t be promoted as they are the "reserve team" of Sporting Cristal which plays in First Division.

League table

Pos	Team	Pld	W	D	L	GF	GA	GD	Pts	Qualification
1	Deportivo Aviación	24	15	7	2	39	14	+25	52	Promotion playoff
2	Alcides Vigo	24	15	3	6	38	23	+15	48
3	Hijos de Yurimaguas	24	14	4	6	30	21	+9	46
4	Sporting Cristal B^{[note 1]}	24	13	3	8	51	32	+19	42
5	América Cochahuayco	24	12	5	7	34	27	+7	41
6	AELU	24	10	8	6	27	18	+9	38
7	Lawn Tennis	24	10	6	8	30	23	+7	36
8	Sport Coopsol	24	7	8	9	26	24	+2	29
9	Unión Huaral	24	7	4	13	22	31	−9	25
10	Bella Esperanza	24	6	4	14	27	64	−37	22
11	Virgen de Chapi	24	4	9	11	25	34	−9	21
12	Olímpico Somos Peru	24	4	6	14	19	37	−18	18
13	Guardia Republicana	24	3	5	16	22	42	−20	14

Updated to match(es) played on 2001. Source: ^{[citation needed]}
Rules for classification: 1) points; 2) goal difference; 3) number of goals scored.

Deportivo Aviación	1–3	Deportivo UPAO

^ As the senior team was competing in Primera División, the club played the second division with reserve teams.