The 1999 Iowa State Cyclones football team represented Iowa State University during the 1999 NCAA Division I-A football season. They played their home games at Jack Trice Stadium in Ames, Iowa. They participated as members of the Big 12 Conference in the North Division. The team was coached by head coach Dan McCarney.

Date	Time	Opponent	Site	TV	Result	Attendance
September 2	7:00 p.m.	Indiana State*	Jack Trice Stadium Ames, IA		W 33–7	33,936
September 11	6:00 p.m.	Iowa*	Jack Trice Stadium Ames, IA (Battle for the Cy-Hawk Trophy)	FSN	W 17–10	50,402
September 18	9:00 p.m.	at UNLV*	Sam Boyd Stadium Whitney, NV		W 24–0	26,176
September 25	11:30 a.m.	No. 15 Kansas State	Jack Trice Stadium Ames, IA (rivalry)	FSN	L 28–35	40,057
October 9	6:00 p.m.	at No. 4 Nebraska	Memorial Stadium Lincoln, NE	FSN	L 14–42	77,743
October 16	6:00 p.m.	at Missouri	Faurot Field Columbia, MO (rivalry)	FSN	W 24–21	61,052
October 23	1:00 p.m.	Colorado	Jack Trice Stadium Ames, IA		L 12–16	34,892
October 30	11:30 a.m.	No. 12 Texas	Jack Trice Stadium Ames, IA	FSN	L 41–44	37,218
November 6	1:00 p.m.	at Texas Tech	Jones Stadium Lubbock, TX		L 16–28	41,691
November 13	1:00 p.m.	Oklahoma	Jack Trice Stadium Ames, IA		L 10–31	37,073
November 20	1:00 p.m.	at Kansas	Memorial Stadium Lawrence, KS		L 28–31	27,000
*Non-conference game Homecoming Rankings from AP Poll released prior to the game All times are in Central time

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