The year 1549 in science and technology included some events, a few of which are listed here.

• November 30 – Henry Savile, English polymath and benefactor (died 1622)
• Michiel Coignet, Flemish engineer, cosmographer, mathematician and scientific instrument-maker (died 1623)
• Nikola Vitov Gučetić, Ragusan polymath (died 1610)

• April – Andrew Boorde, English physician and traveller (born 1490)
• August – Jacob Ziegler, German geographer (born c. 1470/1)